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Journal / Cancer Research / Samodling och transduktion av murina tymocyter på …
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Cancer Research

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JoVE Journal Cancer Research
Samodling och transduktion av murina tymocyter på deltaliknande 4-uttryckande stromaceller för att studera onkogener i T-cellsleukemi
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Samodling och transduktion av murina tymocyter på deltaliknande 4-uttryckande stromaceller för att studera onkogener i T-cellsleukemi

Article DOI: 10.3791/64271-v • 09:43 min • June 9th, 2023
June 9th, 2023 •
1, 1, 1,2,3, 1, 1,2,3, 4, 1, 1
1Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute, National Institutes of Health, 2Comparative Biomedical Scientist Training Program, NIH, 3Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory, Michigan State University, 4NCI-Frederick Laboratory Animal Sciences Program

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Detta protokoll beskriver isoleringen av dubbelnegativa tymocyter från mustymus följt av retroviral transduktion och samodling på det deltaliknande 4-uttryckande benmärgstromala cellinje-samodlingssystemet (OP9-DL4) för ytterligare funktionell analys.

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Cancerforskning utgåva 196 Tymocyt OP9 lymfopoieser transduktion samkultur
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