Methods Collections

CRISPR-Cas9: A new era of targeted gene editing

Open for Submissions
Methods Collections
CRISPR-Cas9: A new era of targeted gene editing

Guest Editors
Sridhar Bammidi

Indian Institute of Technology

Sridhar Bammidi is an ocular neurobiologist, with interests primarily in understanding the developmental biology of the...

Avijit Banik

Emory University

Avijit Banik is a Cognitive Neuroscientist with a specialization in the field of Alzheimer’s disease (AD). The key...

Gillipsie Minhas

Cactus Communications

Gillipsie Minhas earned her Ph.D. in Neurosciences (2016) at the Post Graduate Institute of Medical Education and...

Collection Overview

Gene editing is an inevitable molecular biology tool that is used to alter an organism’s DNA. Over time, a number of techniques have been developed for gene or genome editing such as homologous recombination, zinc-finger nucleases, RNA interference, and others. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (CRISPR-Cas9) is the most recent development in the field of gene editing that has fascinated scientists worldwide. Cas9 is a bacterial nuclease, first isolated from Streptococcus pyogenes, and it is indispensable for the survival of the bacteria against various infections. CRISPR-Cas9 is a simple technique and consists of two components, a nuclease (CAS9) and a guide RNA. The technique has the advantage of being quick, accurate, and more reliable than the other existing counterparts. Moreover, while this technique was originally developed for genome editing, it is now being exploited in transcriptome and metabolic pathways, cell signaling pathways, genotyping, and many other applications.  CRISPR-Cas9 also holds great promise in the prevention and treatment of human diseases. It is being explored to treat different complex and genetic diseases including sickle cell anemia, cancer, hemophilia, cystic fibrosis, HIV infection, neurological and neuromuscular diseases. Furthermore, it can be used in stem cell reprogramming, vaccine development, and disease screening and diagnosis. Through this methods collection we aim to bring together basic biology of the CRISPR-Cas9 technique, its applications as a genome editing tool in different cell lines and animal models, and its potential in disease prevention and treatment.