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Methods Collections

Hepato-Biology and High-Throughput Techniques Applied to Liver Research

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Methods Collections
Hepato-Biology and High-Throughput Techniques Applied to Liver Research

Guest Editors
Teresa Rubio-Tomás

Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas

Teresa received her Bachelor’s and Master’s in degree Biology and Biomedical Research respectively from the…

Ana I. Álvarez-Mercado

University of Granada (Spain), Department of Biochemistry and Molecular Biology

With a degree in Chemistry, Ana started her research career at Biochemistry of Nutrition Therapeutic Implication,…

Ascensión Rueda Robles

Campus Cartuja University of Granada, Department of Nutrition and Bromatology

Dr. Rueda holds a degree in Human Nutrition and Dietetics (2009), a degree in Food Science and Technology (2011). She…

Ruben Lopez-Aladid

University of Barcelona

Ruben works on infections and respiratory diseases at the Hospital Barcelona Clinic.

Throughout his scientific…

Evelina Charidemou

University of Cyprus

Evelina Charidemou is a Marie Skłodowska-Curie fellow studying the intimate link of metabolism and epigenetics in…

Collection Overview

Liver diseases pose a major health concern globally. Although, there are genetic factors that mainly account for autoimmune liver disease, in most cases, they are the consequence of chronic liver injury due high fat diet, excessive alcohol intake, hepatitis B and C virus infection, and long-term exposure to hepatotoxic drugs, food contaminators or environmental chemicals.

Chronic liver injury can progress from cirrhosis and liver failure to liver cancer, ultimately. Diagnostic and treatment options are limited and the availably of healthy livers for transplantation is ultimate solution but poses multiple problems.

Thus, studying liver biology in health and disease is fundamental for a better understanding of the physiopathological processes underlying liver diseases and for translation into better clinical options.

Therefore, in this collection we would like to cover both the aspects of hepato-biology i.e., isolation of liver cell types, and high-throughput techniques applied to study liver biology and pathophysiology, i.e., single cell experiments, ChIP-seq, ATAC-seq, metabolomics/lipidomics, and other state-of-the art techniques. Liver cells isolation and culture is not trivial and the maintenance of the phenotype in culture and/or after freezing is challenging. Here, we aim to help researchers to work with all liver cell types i.e. hepatocytes, biliary epithelial cells, as well as Kupffer cells, neutrophils, and other immune cells in different experimental set ups.


Nuclei Isolation from Fresh Frozen Liver Tissue for Single-Cell Multi-omics.

Mateusz Strzelecki1, Celia Martinez Jimenez*1, Ioannis Konstantinos Deligiannis1, Kelvin Yin1
1Helmhotlz Pioneer Campus, Helmholtz Zentrum München

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