Liver diseases pose a major health concern globally. Although, there are genetic factors that mainly account for autoimmune liver disease, in most cases, they are the consequence of chronic liver injury due high fat diet, excessive alcohol intake, hepatitis B and C virus infection, and long-term exposure to hepatotoxic drugs, food contaminators or environmental chemicals.

Chronic liver injury can progress from cirrhosis and liver failure to liver cancer, ultimately. Diagnostic and treatment options are limited and the availably of healthy livers for transplantation is ultimate solution but poses multiple problems.

Thus, studying liver biology in health and disease is fundamental for a better understanding of the physiopathological processes underlying liver diseases and for translation into better clinical options.

Therefore, in this collection we would like to cover both the aspects of hepato-biology i.e., isolation of liver cell types, and high-throughput techniques applied to study liver biology and pathophysiology, i.e., single cell experiments, ChIP-seq, ATAC-seq, metabolomics/lipidomics, and other state-of-the art techniques. Liver cells isolation and culture is not trivial and the maintenance of the phenotype in culture and/or after freezing is challenging. Here, we aim to help researchers to work with all liver cell types i.e. hepatocytes, biliary epithelial cells, as well as Kupffer cells, neutrophils, and other immune cells in different experimental set ups.