Methods for elucidating action mechanisms of immunotherapies
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Vandana Kaul is an immunologist and is interested in understanding the mechanisms of action of immunotherapies for…
Immune effector mechanisms are a critical component of maintaining homeostasis in living systems at multiple levels. Various components of the immune system come together to protect the host during a pathological attack. Immune mechanisms then challenge, address, rectify, and quarantine vital body functions and systems in a highly specific and context-dependent manner. A major achievement of the past century has been the ability to harness and augment this existing system with potent extraneous (but similar) molecules such as antibodies in order to achieve great advances in both disease control and cure. With multiple such antibody-therapeutics already approved and hundreds in pipeline, the importance of understanding the intricate mechanisms by which these therapies work cannot be overstated. This collection focuses on the following areas of interest: (1) Characterization of drug responses in primary immune cell populations to understand signaling mechanisms of activation/activation, proliferation, and immune-mediated cytotoxicity; (2) Action mechanisms of antibody mediated therapeutics (ADCC, ADCP, CDC, etc.); (3) Novel methods for techniques in flow cytometry, imaging, or in vitro functional assays to understand immune-effector functions; (4) Novel cell-based assays for immunological targets (especially Immuno-oncology targets) that are predictive of in vivo biology in order to identify novel therapeutics for immunotherapy; and (5) Methods for Single-cell analysis for understanding heterogeneity, poly-functionality, and quasi-states of immune cells.