Methods for studying CRISPR-Cas systems
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Institute of Microbiology
I’m a postdoctoral researcher in Agata Krawczyk-Balska’s lab at University of Warsaw. My current research...
Due to their unique mode of action, CRISPR-Cas systems employ multiple sophisticated mechanisms. These include numerous interactions between various types of molecules (e.g., RNA, DNA, and proteins). Precise description of these interactions is crucial both for basic studies as well for designing practical applications for CRISPR-Cas systems of various types. Yet, it is not an easy task to adapt existing methods to achieve reliable results in this uneasy field. The aim of this collection is to gather methods developed and optimized for studying CRISPR-Cas systems at each stage of their activity: spacer acquisition, biogenesis, and interference.