Guest Editor
Collection Overview
Profiling the biomolecular composition and morphological structure of cells and tissues in a non-perturbing manner is crucial in the life sciences field. Label-free microscopy, through light-matter interactions, offers a non-invasive and quantitative characterization tool for live imaging and translational biomedicine by revealing native and pristine traits of analytes. The current frontier in label-free biomedical imaging involves combining diverse methods into multimodal platforms to achieve comprehensive morpho-molecular profiling through engineered multi-channel illumination and detection schemes.
Such combined imaging methods include, but are not limited to:
1. Vibrational micro-spectroscopy:
- Infrared (IR) spectroscopy
- Raman spectroscopy
- Brillouin spectroscopy
- Coherent Anti-Stokes Raman Scattering (CARS)
- Stimulated Raman Scattering (SRS)
- Coherent Stokes Raman Scattering (CSRS)
2. Quantitative phase imaging (QPI):
- Digital holography
- Holo-tomography
3. Optical coherence tomography (OCT)
4. Single- and multi-photon imaging:
- Two-Photon Excited Fluorescence (2PEF)
- Three-Photon Excited Fluorescence (3PEF)
- Second Harmonic Generation (SHG)
- Third Harmonic Generation (THG)
This JoVE Methods Collection will explore the latest implementations of label-free multimodal imaging, highlighting the potential of advanced multimodal systems to revolutionize biological research and biomedical practice.
Articles
Multimodal Optical Imaging Platform for Studying Cellular Metabolism
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2025
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1Shu Chien-Gene Lay Department of Bioengineering, University of California San Diego, 2Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California San Diego, 3Department of Electrical and Computer Engineering, University of California San Diego, 4Institute of Engineering in Medicine, University of California San Diego, 5Synthetic Biology Institute, University of California San Diego
A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells
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2025
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Leonardo Bianchi1,2,3, Arianna Bresci2, Koseki J. Kobayashi-Kirschvink2,4, Gabriela Paroni5, Paola Saccomandi3, Peter T. C. So1,2,6, Jeon Woong Kang2
1Department of Mechanical Engineering, Massachusetts Institute of Technology, 2Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, 3Department of Mechanical Engineering, Politecnico di Milano, 4Klarman Cell Observatory, Broad Institute of MIT and Harvard, 5Department of Molecular Biochemistry and Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 6Department of Biological Engineering, Massachusetts Institute of Technology
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
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Cited by 3
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2025
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Kevin K. D. Tan1,2, Alejandro De la Cadena1, Edita Aksamitiene1, Alexander Ho1,2, Stephen A. Boppart1,2,3,4
1Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, 2Department of Bioengineering, University of Illinois Urbana-Champaign, 3Department of Electrical and Computer Engineering, University of Illinois Urbana-Champaign, 4NIH/NIBIB Center for Label-free Imaging and Multiscale Biophotonics, University of Illinois Urbana-Champaign
