Mycobacterial pathogens, especially M. tuberculosis, reportedly infect one-third of the world’s population and cause nearly two million deaths annually. Additionally, the recent surge in multidrug-resistant mycobacterial strains poses ongoing challenges for tuberculosis drug therapies and diagnosis. Thus, there is a growing need for effective strategies for mycobacterial gene manipulation.  

This Methods Collection focuses on protocols for handling and manipulating Mycobacteria and their genes. It aims to serve as a comprehensive resource for cutting-edge methods in mycobacterial research, promoting knowledge sharing and encouraging interdisciplinary collaboration. The collection will assist researchers in preparing and implementing efficient studies while fostering innovative solutions to address critical public health issues posed by tuberculosis infection.