
Kajal Kanchan
Amity Institute of Molecular Medicine and Stem Cell Research, Amity University Uttar Pradesh, Noida, India
<p><span style="color: rgb(0, 0, 0);">Dr. Kajal Kanchan obtained PhD in 2011 from University of Tübingen, Germany in the area of Mycobacterial cell signaling and pathogenesis. Subsequently, she worked as a Marie Curie Postdoctoral fellow at University of Debrecen, Hungary, and characterized Human transglutaminase 2 protein, biochemically and structurally. Later, she moved to University of Cambridge, UK, and worked with histone variants and got an exposure in field of transcription and gene regulation. With such diverse background and experience, she established her research niche focusing on gene regulation in mycobacterial pathogenesis. She received the Ramalingaswami Re-entry fellowship from DBT (Department of Biotechnology, Government of India) to work in the area of temperature signaling in mycobacterium and worked at School of Biotechnology, Jawahar Lal University, New Delhi. Later, she moved to Amity Institute of Molecular Medicine and Stem Cell research, Amity University, Noida. The broad goal of her research team is to explore how bacteria sense and respond to changes in the environment. Her team is using </span><em style="color: rgb(0, 0, 0);">M. tuberculosis/M. smegmatis</em><span style="color: rgb(0, 0, 0);"> as model organism and trying to decipher the molecular basis of perception and signal transduction and how these regulate the development and virulence of the Mycobacteria. To achieve these goals she is using genomics, proteomics and computational approaches to model transcriptional, post-transcriptional and translational regulation of the pathogen to find novel intervention measures to treat tuberculosis effectively. </span></p>
Manoj Kumar
Amity Institute of Genome Engineering, Amity University, Noida, India
<p class="ql-align-justify">Dr. Manoj Kumar did his Ph.D. from Max Planck Institute and University of Cologne, Cologne, Germany in Natural Science. He has nearly 15 years of post-PhD experience with four years of postdoctoral training at University of Cambridge, UK. He has extensive experience in molecular biology, Genetics, biochemistry, cell biology, developmental biology as well as next generation sequencing (NGS) data analysis like DNA/RNA-Seq, ChIP-Seq etc. During his PhD and post-doctoral studies, he has performed several genetic screens to identify novel genes leading to developmental plasticity in eukaryotes in response to external cues. He has published papers in leading journals like Science, Cell reports, EMBO reports, Genetics etc. </p><p class="ql-align-justify"> </p><p class="ql-align-justify">Presently, he is a professor at Amity institute of Genome Engineering and his group is working in the area of stress/signal perception and response mechanism of organism in response to external cues. </p>
Mycobacterial pathogens, especially M. tuberculosis, reportedly infect one-third of the world’s population and cause nearly two million deaths annually. Additionally, the recent surge in multidrug-resistant mycobacterial strains poses ongoing challenges for tuberculosis drug therapies and diagnosis. Thus, there is a growing need for effective strategies for mycobacterial gene manipulation.
This Methods Collection focuses on protocols for handling and manipulating Mycobacteria and their genes. It aims to serve as a comprehensive resource for cutting-edge methods in mycobacterial research, promoting knowledge sharing and encouraging interdisciplinary collaboration. The collection will assist researchers in preparing and implementing efficient studies while fostering innovative solutions to address critical public health issues posed by tuberculosis infection.
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2025
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Jennifer Williams*1, Janré Steyn*1, Emilyn Costa Conceição*1, Felicia Bernita Wells1, Melanie Grobbelaar1, Nabila Ismail1, Yonas Ghebrekristos1,2, Christoffel Johannes Opperman1,2,3,4, Sarishna Singh1,2, Jason Limberis5, Fahd Naufal5, Brendon Coenrad Mann1, Rebecca E. Colman6,7, Timothy Rodwell*6,7, Robin Mark Warren*1, John Z. Metcalfe*5
1Division of Molecular Biology and Human Genetics, SAMRC Centre for TB Research, DSI-NRF Centre of Excellence for Biomedical TB Research, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, 2National Health Laboratory Service, Green Point Tuberculosis Laboratory, 3Division of Medical Microbiology, Department of Pathology, University of Cape Town, 4Division of Medical Microbiology, Department of Pathology, University of Cape Town, 5Division of Pulmonary and Critical Care Medicine, Zuckerberg San Francisco General Hospital and Trauma Centre, University of California, San Francisco, 6Department of Medicine, University of California, San Diego, School of Medicine, 7Foundation for Innovative New Diagnostics (FIND), Campus Biotech
Site-directed mutagenesis in mycobacteria
Christian Otum1
1Stellenbosch University
DNA extraction from Mycobacteria species using CTAB method
Shatha Sultan Ahmed Omar*1
12DST/NRF Centre of Excellence for Biomedical Tuberculosis Research/ SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa
Semi-automated quantification of 18F-FDG PET-CT in pulmonary TB contacts and its association with prospective outcomes.
Jee Whang Kim*1,
Joanne Lee1,
Ismail Novsarka1,
Anver Kamil2,
Pranabashis Haldar*1
1University of Leicester,
2University Hospitals of Leicester NHS Trust
Adapting the Deeplex® Myc-TB Kit for Tuberculosis Drug Resistance Profiling Using The Oxford Nanopore MinION
Brendon Mann*1
1Stellenbosch University
Mycolic acid extraction and analysis
Labeeqah Harris1
1Stellenbosch University