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Methods Collections

Current tools and methods in taste science

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Methods Collections
Current tools and methods in taste science

Guest Editors
Dany Gaillard

University of Colorado Anschutz Medical Campus

Dr. Dany Gaillard is an instructor in the Department of Cell and Developmental Biology at the University of Colorado…

Collection Overview

The sense of taste is essential to preserve a healthy body and mind. While taste contributes to the selection of beneficial compounds over noxious compounds to ensure proper nutrition and prevent intoxication, it is also involved in the pleasure associated with food intake. However, taste perception can be diminished (partially or fully) or altered by a variety of insults, including cancer treatments, infections, nerve damage and other ailments, which can be detrimental to health. Taste loss is associated with malnutrition, weight loss, depression and social isolation. Therefore, understanding how taste buds develop, how they are maintained, how tastants are detected, how the gustatory signal is transduced and processed in the periphery and the central nervous system, how the body responds to taste stimuli and how taste perception changes is key to establish approaches to prevent or mitigate taste loss and disruption.

Exploration of the taste system has made considerable progress thanks to the use of a broad array of tools that are constantly evolving and embracing cutting-edge technologies. The goal of this JoVE Methods Collection is to document those tools and methods in one location to help taste scientists find the resources they need. Examples of methods to be included can be related to signal transduction, gene expression (from PCR to sequencing), cell/nerve/ganglion isolation and recording, central integration of the gustatory signal, microscopy, development and maintenance of the taste system, taste cell/organoid cultures, in vivo imaging, animal behavior, and psychophysical studies.

Abstracts

μTongue: microfluidics-based functional imaging platform for the tongue in vivo

Myunghwan Choi*1, Jisoo Han2, Pyonggang Choi3
1School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea, Center for Neuroscience Imaging Research, Institute for Basic Science, Suwon 16419, Republic of Korea, 2Department of Biomedical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea, Center for Neuroscience Imaging Research, Institute for Basic Science, Suwon 16419, Republic of Korea, 3School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea

Generation and culture of lingual organoids derived from adult mouse taste progenitor cells

Lauren Shechtman*1, Christina Piarowski*1, Jennifer Scott1, Dany Gaillard*1, Linda Barlow*1
1The University of Colorado Anschutz Medical Campus

Psychophysical Tracking Method to Measure Taste Detection Thresholds in Children, Adolescents and Adults

Paule Joseph*1, Julie Mennella*2, M. Yanina Pepino*3
1National Institute of Nursing Research, Sensory Science and Metabolism Unit, Bethesda, MD, USA, 2Monell Chemical Senses Center, Philadelphia, PA, USA, 3University of Illinois at Urbana Champaign, Department of Food Science and Human Nutrition and Division of Nutritional Sciences, Urbana, IL, USA

Whole Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds.

Lisa Ohman*1, Robin Krimm*1
1University of Louisville

Purification and characterization of fat sensor-positive taste bud cells from mouse tongue papillae

Aziz Hichami*1, Amira Sayed Khan1, naim Akhtar Khan1
1INSERM U1231, Université de Bourgogne Franche Comté (UBFC), Dijon, France

A method for in vivo calcium imaging of mouse geniculate ganglion neuron responses to taste stimuli.

Lindsey Macpherson*1, Bryan Fowler1
1The University of Texas at San Antonio

Cell dissociation from tongue epithelium and mesenchyme of E12.5 and 8-wk-old mice

Hongxiang Liu*1
1Regenerative Bioscience Center, Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia

A streamlined food-preference assay in Drosophila

Yali Zhang*1, John Mack2
1Monell Chemical Senses Center, Department of Physiology, The Diabetes Research Center, University of Pennsylvania Perelman School of Medicine, 2Monell Chemical Senses Center

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