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Methods Collections

iNEXT-Discovery: Integrating high-end facilities for translational research in structural biology

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Methods Collections
iNEXT-Discovery: Integrating high-end facilities for translational research in structural biology

Guest Editors
Anastassis Perrakis

Netherlands Cancer Institute

Dr. Anastassis Perrakis is a Group Leader in Structural Biology in the Division of Biochemistry at the Netherlands Cancer …

Hans Wienk

Netherlands Cancer Institute

Dr. Hans Wienk is a Project Manager in the Division of Biochemistry at the Netherlands Cancer Institute (NKI) in…

Collection Overview

Integrative structural biology uses a large variety of biophysical methods that provide detailed knowledge of the atomic and molecular structure of macromolecules, isolated or in their native environment. This provides a better understanding of the basic of chemistry of life and helps with the development of new biotechnology tools, drugs, and biomaterials. Access to large-scale installations (synchrotrons producing X-rays, state-of-the-art electron microscopes, powerful NMR magnets) is necessary for research in structural biology. This Methods Collection will describe the most important methods for the structural analysis of macromolecules, applications in fragment-based lead drug discovery, methods to investigate the structure of macromolecules in their native environment, and high throughput approaches for structural determination. It will consist of a variety of methods that includes, but is not limited to, NMR, cryo-EM, X-ray crystallography, and scattering.

Articles

Abstracts

nano-Differential Scanning Calorimetry for fragment-based lead discovery

Anastassis Perrakis*1
1Netherlands Cancer Institute

A sample preparation pipeline for microcrystals at the VMXm beamline

Adam Crawshaw*1, Emma Beale1, Anna Warren1, Andrew Stallwood1, Graham Duller1, Jose Trincao1, Gwyndaf Evans*1
1Diamond Light Source Ltd

Single particle cryo-electron microscopy- a guide, from sample to structure

Rebecca Thompson*1
1University of Leeds

The XChem facility at Diamond for screening 1000 fragments by crystallography per week

Alice Douangamath*1, Ailsa Powell1, Daren Fearon2, Romain Talon1, Patrick Collins1, Jose Brandao-Neto1, Louise Dunnett1, Alexandre Dias1, Anthony Aimon1, Tobias Krojer3, Nicholas Pearce1, Conor Wild1, Frank von Delft*1
1Diamond Light Source, 2Diamond Light source, 3Structure Genomic Consortium - Oxford

NMR-based fragment screening in a minimum sample but maximum automation mode

Harald Schwalbe*1, Hannes Berg1, Maria Wirtz Martin1, Christian Richter1, sridhar Sreeramulu1
1Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt/M., Germany.

Serial Helical Line Scan implementation of serial crystallography at cryogenic and ambient temperatures (on EMBL beamline P14 on PETRA III)

Thomas R. Schneider*1, Johanna Hakanpää2, Gleb Bourenkov1
1European Molecular Biology Laboratory, Hamburg Unit c/o DESY, Building 25A, Notkestrasse 85, 22607 Hamburg, Germany, 2DESY, Notkestr. 85, 22607 Hamburg, Germany

A sample preparation pipeline for microcrystals at the VMXm beamline

Adam Crawshaw*1, Emma Beale1, Anna Warren1, Andrew Stallwood1, Graham Duller1, Jose Trincao1, Gwyndaf Evans*1
1Diamond Light Source Ltd

CryoEM image processing: From experimental data to 3D maps

David Maluenda*1
1CNB CSIC Spain

Efficient setup of large-scale automated cryo-electron tomography data acquisition

Simone Mattei*1
1European Molecular Biology Laboratory, Structural and Computational Biology Unit, 69117, Heidelberg, Germany

A cartographic description of the cellular landscape by cryo soft X-ray tomography

Eva Pereiro*1, Johannes Groen1, Andrea Sorrentino1, Lucía Aballe1, Robert Oliete1, Ricardo Valcárcel1, Ana Joaquina Pérez-Berná1
1MISTRAL beamline, Experiments Division, ALBA light source

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

Enrico Luchinat*1, Letizia Barbieri1, Lucia Banci1
1Magnetic Resonance Center - CERM, University of Florence, Italy

Online Crystallography: Automated, Remote Controlled Protein-to-Structure Pipelines

Jose Antonio Marquez*1
1European Molecular Biology Laboratory

3D Structured Illumination Microscopy for Cryogenically Preserved Cell Populations for Correlative Cryo-Imaging

Maria Harkiolaki*1
1Diamond Light Source

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