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Methods Collections > Mass spectrometry methodologies for biomolecule analyses

Azad Eshghi

Affiliation: University of Victoria

Dr. Azad Eshghi received a Ph.D. degree in the year 2012 from the University of Victoria - Victoria, British Columbia, Canada. He gained additional research experience as a post-doctoral fellow at the Pasteur Institute- Paris, France at the University of Toronto - Toronto, Ontario, Canada. In the year 2017, Azad joined the team at the University of Victoria Genome BC Proteomics Centre, where he is currently a research associate. 

Dr. Eshghi’s current research focus is on assay development for repeatable quantification of proteins using targeted mass spectrometry methodologies. To achieve high sensitivity and reproducibility, assays are developed using multiple reaction monitoring (MRM) LC-MS/MS utilizing stable isotope-labeled peptide standards for measuring protein concentration. Assay development using MRM includes determination of the lower limit of quantification for each protein target, assessment of multi-day repeatability, matrix effect, selectivity, and stability testing. The MRM assays are developed for measuring protein concentration in dried blood spots, which is an alternative microsampling method, amenable for biomarker discovery and application in screening assays. 


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