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Methods Collections > Molecular and physiological approaches for studying plant-microbe interactions

Alexandre Tromas

Affiliation: Center for Genomic Sciences (formerly CIFN) at the UNAM

Alexandre Tromas studied Plant biology during his undergrad at the University of Tours, France. He did his doctoral studies at the CNRS (National Center for Scientific research), about the role of the Auxin binding protein 1 ABP1 in auxin signaling and root development in Arabidopsis thaliana under the supervision of Catherine Perrot-Rechenmann (CNRS). He received his doctoral title from the University Paris Sud 11-Saclay in 2010. As an IRD (Institute for research in Developing countries) Fellow, he did postdoctoral work in Dakar, Senegal at the LCM (Shared laboratories: UCAD/ISRA/IRD) with Laurent Laplaze (IRD) working on genomics and comparative transcriptomics of actinorhizal symbiosis between the tropical tree, casuarina glauca and the nitrogen-fixing bacteria Frankia.
As a NSERC (Natural Sciences and Engineering Research Council of Canada) Fellow, he did postdoctoral work in London, Canada at the AAFC (Agriculture and Agri-Food Canada) with Krzysztof Szczyglowski (AAFC) working on evaluating the developmental processes taking place in the model legume Lotus japonicus, in response to accommodation of the nitrogen-fixing bacteria, Mesorhizobium loti.
He currently holds the position of an Assistant Professor (Investigador Asociado C) at the Center for Genomic Sciences (formerly CIFN) at the UNAM (Campus Morelos, Cuernavaca). Alexandre’s current research project is focusing on the first steps of the establishment of the NFS and consists in identifying and characterizing signaling complexes triggering cytoskeleton rearrangement in response to symbiotic bacteria in Lotus japonicus. To do so, I have developed two approaches, in parallel, to better characterize proteins of the Rho-GTPase family (ROP in plants), playing a central role in this process. The first strategy consists in determining the changes of ROP Interactome following inoculation with the symbiotic bacteria. The second involves the development of a biosensor to draw a spacio-temporal map of the site of activation of the ROP proteins in the root hair, site of the symbiotic bacteria entry. This combined use of molecular biology, biochemistry and cell biology should result in a better understanding of how the plant reorganizes its own physiology to accommodate the bacterial symbiont.

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