High-throughput Automation

High-throughput automation is the use of automated systems to perform, coordinate, and analyze large numbers of experimental or manufacturing operations with consistent timing and handling. In bioengineering, robotic liquid handlers, plate-based workflows, sensors, and software transfer samples between steps, while standardized protocols and data capture reduce manual variation and accelerate processing. These systems support rapid screening of biomolecules, cells, materials, and process conditions, enabling researchers to compare many experimental variables in parallel. By increasing reproducibility and experimental scale, high-throughput automation helps advance drug discovery, synthetic biology, diagnostics, tissue engineering, and bioprocess development.

High-throughput Automation - Related Videos

Research

JoVE Journal - Biology
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High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

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Cited by 5 •

2012

Genome assemblies based on massively parallel DNA sequencing technologies are usually highly fragmented. The development of physical chromosome maps can potentially improve genome assemblies. Here, we demonstrate innovative approaches to chromosome preparation, fluorescent in situ hybridization, and imaging that significantly increase throughput of the physical map development.

Research

JoVE Journal - Bioengineering

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

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Cited by 48 •

2016

We present a protocol on how to utilize high-throughput cryo-electron tomography to determine high resolution in situ structures of molecular machines. The protocol permits large amounts of data to be processed, avoids common bottlenecks and reduces resource downtime, allowing the user to focus on important biological questions.

Research

JoVE Journal - Neuroscience
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Automated High-throughput Behavioral Analyses in Zebrafish Larvae

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Cited by 29 •

2013

Our laboratory developed a novel high-throughput automated imaging system that is useful for the detection of several different behaviors in 7-day-old zebrafish larvae. The system can be used for detecting subtle changes in behavior after the larvae have been exposed to environmental toxicants or pharmaceuticals.

Research

JoVE Journal - Biology
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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

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Cited by 25 •

2012

We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C. elegans.

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

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2025

This report describes an automated protocol for multiplex immunofluorescence (mIF) assays on an automated slide stainer. It demonstrates the high reproducibility of spatial proteomics on standard high-throughput tissue autostainers and whole-slide fluorescence imagers, which is ideal for custom mIF assays across a large number of tissue slides in translational research.

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