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JoVE Journal
Bioengineering

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Using Tomoauto
 

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Article DOI: 10.3791/53608
January 30th, 2016

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Summary January 30th, 2016

We present a protocol on how to utilize high-throughput cryo-electron tomography to determine high resolution in situ structures of molecular machines. The protocol permits large amounts of data to be processed, avoids common bottlenecks and reduces resource downtime, allowing the user to focus on important biological questions.

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