Subcutaneous Xenograft
Subcutaneous Xenograft - Related Videos
10:35
Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice
0 Views •
Cited by 15 •
2013
Human tumor xenografts in immunodeficient mice are valuable tools to study cancer biology. Specific protocols to generate subcutaneous and intrahepatic xenografts from human hepatocellular carcinoma cells or tumor fragments are described. Liver regeneration induced by partial hepatectomy in recipient mice is presented as a strategy to facilitate intrahepatic engraftment.
08:38
Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia
0 Views •
Cited by 13 •
2013
We describe the manufacture of compressed hormone pellets, as well as subcutaneous surgical implantation into mice. This strategy can be combined with the growth of cell and tissue xenografts under the renal capsule of athymic nude mice to evaluate hormonal carcinogenesis and regulation of benign prostate growth.
11:44
Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
0 Views •
Cited by 40 •
2010
Cancer stem cells (CSCs) have been identified in a number of malignancies. In this protocol we describe a flow cytometric method utilizing aldehyde dehydrogenase activity and CD44 and CD24 expression to isolate CSCs from human pancreatic adenocarcinoma xenografts. These viable cells can then be used in functional and analytical studies.
02:26
A Technique to Generate a Human PBMC-Engrafted Humanized Xenograft Mouse Model
0 Views •
2025
This video demonstrates an assay to generate a humanized xenograft mouse model. An immunocompromised mouse is injected with cyclophosphamide to cause immune cell depletion. Patient-derived tumor cells mixed with human peripheral blood mononuclear cells (PBMCs) in a hydrogel are subcutaneously injected into the mouse, forming a tumor with infiltrating T cells.
10:41
Germ Cell Transplantation and Testis Tissue Xenografting in Mice
0 Views •
Cited by 16 •
2012
Protocols for germ cell transplantation and testis tissue xenografting are described. Germ cell transplantation results in donor-derived spermatogenesis in recipient testes and represents a functional reconstitution assay for identification of spermatogonial stem cells (SSCs). Testis tissue xenografting reproduces testis development and spermatogenesis of various donor species in recipient mice.
FAQs
The surrounding extracellular matrix provides the local context in which implanted cells or tissue interact with the host. These interactions can support establishment of a localized graft, tissue formation, or tumor mass. In bioengineering studies, observing how the graft develops within this environment helps researchers assess whether a material or engineered construct supports appropriate integration and biological responses.
Immunocompromised hosts are used because they can support engraftment of living cells or tissue from another species. This condition helps the implanted material establish a localized graft, allowing investigators to examine growth, tissue development, or therapeutic response in vivo. The host’s biological environment still influences the graft, so the model provides information beyond what can be observed in isolated culture.
Researchers can monitor graft growth, vascularization, biocompatibility, and therapeutic response. Growth indicates how the implanted material develops over time, while vascularization reflects formation of blood supply around the graft. Biocompatibility concerns how the construct interacts with its host environment. Together, these observations help determine whether a candidate therapy or engineered tissue merits evaluation in more complex models.
The workflow begins by placing living cells or tissue beneath the skin of a host, commonly one capable of supporting engraftment. The implanted material then establishes a localized graft as it interacts with the surrounding environment. Subsequent observation focuses on graft growth, vascularization, biocompatibility, and therapeutic response, generating in vivo evidence for the tested construct or treatment.
This model can be used to evaluate biomaterials, engineered tissues, drug delivery systems, and cell-based therapies. Each candidate can be examined in relation to graft development and its interaction with the host. The approach is therefore useful when researchers need in vivo information about biological compatibility, tissue formation, delivery-related effects, or treatment response before pursuing more complex models.
Placement beneath the skin creates an accessible graft location that supports monitoring during the study. Researchers can follow changes in growth, vascularization, biocompatibility, and therapeutic response without beginning with a more complex model. This accessibility makes the approach valuable as an intermediate evaluation step for engineered tissues, biomaterials, drug delivery systems, and cell-based therapies.