Vesicle Size
Vesicle Size - Related Videos
12:01
Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing
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Cited by 99 •
2014
Extracellular vesicles play important roles in physiological and pathological processes, including coagulation, immune responses, and cancer or as potential therapeutic agents in drug delivery or regenerative medicine. This protocol presents methods for the quantification and size characterization of isolated and non-isolated extracellular vesicles in various fluids using tunable resistive pulse sensing.
04:38
A Size Exclusion Chromatography Technique to Purify Mycobacterial Extracellular Vesicles
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2025
This video demonstrates the size exclusion chromatography (SEC) technique for purifying bacterial extracellular vesicles (EVs) from the culture filtrate of Mycobacterium tuberculosis (Mtb). The technique utilizes a porous resin-packed column to obtain purified fractions of EVs. The porous particles selectively allow smaller proteins to enter the pores, resulting in slower elution. At the same time, EVs are excluded from entering the pores due to their larger size and are eluted first.
04:51
Size Exclusion Chromatography Based Separation of Bacterial Extracellular Vesicles: A Technique to Separate Heterogenous Population of Gram-Negative Bacterial Outer Membrane Vesicles Based on Their Size
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2025
In this video, we demonstrate a technique–size exclusion chromatography–to separate a heterogenous population of outer membrane vesicles or OMVs derived from bacteria. These vesicles are nanosized spherical structures and are separated into large and small subpopulations based on their size through Size Exclusion Chromatography.
11:35
Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles
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Cited by 29 •
2012
This protocol describes an extrusion method for preparing lipid vesicles of sub-micron sizes with a high degree of homogeneity. This method uses a pressure-controlled system with controlled nitrogen flow rates for liposome preparation. The lipid preparation1,2, liposome extrusion, and size characterization will be presented herein.
03:24
Isolation of Bacterial Extracellular Vesicles Using Size-Exclusion Chromatography
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2025
Source: Watson, D. C., et al. Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria. J. Vis. Exp. (2021)This video demonstrates the use of size exclusion chromatography with porous resin beads to isolate bacterial extracellular vesicles (EVs) from a bacterial culture filtrate. Larger EVs elute early by bypassing the resin pores, while smaller proteins enter the pores and elute later.
FAQs
Coat proteins help shape the membrane as a vesicle buds, while lipid composition affects the membrane’s physical behavior during bending and fission. Together, these factors contribute to the dimensions reached before separation. Examining their influence helps researchers connect molecular control of membrane remodeling with the size of transport or storage compartments formed inside cells.
The amount of cargo can influence the final dimensions of a vesicle because the membrane must accommodate the material being transported, stored, or processed. Size therefore reflects more than membrane remodeling alone. Relating cargo load to vesicle dimensions can help explain how cells organize intracellular trafficking and distinguish changes in compartment formation from changes in transported contents.
Cytoskeletal forces participate in the physical processes that bend, move, and separate cellular membranes. Their contribution can affect how membrane buds develop and the dimensions retained after fission. Considering these forces alongside coat proteins, lipids, and cargo provides a broader mechanism for understanding why vesicle dimensions vary during intracellular trafficking rather than treating size as an isolated measurement.
Comparing vesicle dimensions helps distinguish compartments such as endosomes, lysosomes, and secretory vesicles. These measurements provide structural information about how cells separate transport, storage, and processing functions. When dimensions change, the result can also point to altered membrane dynamics or trafficking organization, making size analysis useful for interpreting cellular architecture in biology.
Researchers assess vesicle size by determining a compartment’s diameter or volume and then using that information to compare cellular structures or vesicle systems. The measurement can support identification of endosomes, lysosomes, and secretory vesicles and can reveal differences in organization. Its value depends on interpreting dimensions in relation to membrane transport and compartment function.
Size analysis is useful when researchers investigate extracellular vesicles, membrane dynamics, or disease-related changes. Measurements can reveal shifts in vesicle characteristics and provide a structural readout of altered cellular organization. In this context, dimensions complement broader biological observations by helping connect membrane behavior and trafficking changes with cellular or disease-associated states.
Vesicle dimensions are relevant when designing and evaluating vesicle-based drug delivery systems. Measuring size helps researchers characterize the resulting vesicles and assess whether a design produces the intended physical properties. This application extends size analysis beyond naturally occurring compartments, using membrane-bound carriers as engineered systems for studying and developing delivery approaches.