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Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

DOI:
10.3791/51623-v

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12:01 min

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October 19, 2014

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, ,
1Department of Neurosurgery,University Medical Center Utrecht, 2Brain Center Rudolf Magnus,University Medical Center Utrecht

Chapters

  • 00:05Title
  • 01:48Preparations
  • 03:54Calibrating the Settings
  • 05:20Measuring the Calibration and Sample Particles
  • 07:32Data Analysis
  • 08:15Alternative Approach to Measuring Non-purified Extracellular Vesicles
  • 09:58Results: U87-MG/EGERvIII Extracellular Vesicle Characterization
  • 11:03Conclusion

Summary

Automatic Translation

Automatic Translation

Extracellular vesicles play important roles in physiological and pathological processes, including coagulation, immune responses, and cancer or as potential therapeutic agents in drug delivery or regenerative medicine. This protocol presents methods for the quantification and size characterization of isolated and non-isolated extracellular vesicles in various fluids using tunable resistive pulse sensing.

Tags

Keywords: Extracellular VesiclesMicrovesiclesExosomesTunable Resistive Pulse SensingQNano SystemNanoparticle DetectionSize ProfilingQuantificationBiological FluidsReal-time Calibration

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