Zebrafish Diabetes Model
Zebrafish Diabetes Model - Related Videos
10:03
A Zebrafish Model of Diabetes Mellitus and Metabolic Memory
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Cited by 69 •
2013
Metabolic memory is the phenomenon by which diabetic complications persist and progress unimpeded even after euglycemia is achieved pharmaceutically. Here we describe a diabetes mellitus zebrafish model which is unique in that it allows for the examination of the mitotically transmissible epigenetic components of metabolic memory in vivo.
04:26
Streptozocin Treatment: A Zebrafish Model of Type 1 Diabetes Mellitus
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2023
This video describes the technique of creating a zebrafish model for Type 1 diabetes mellitus with the help of the drug Streptozocin, to study the pathophysiology of diabetic complications and metabolic memory.
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
10:43
Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
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Cited by 98 •
2007
Our protocol was developed to cleanly and easily deliver islets or cells under the kidney capsule of mice. Cells are concentrated into pellets in the final tubing used for transplanting the cells under the kidney capsule. The ease of this technique reduces stress to the cells and the mouse.
13:35
Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules
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Cited by 25 •
2017
This work describes methods to establish acute and chronic hyperglycemia models in zebrafish. The aim is to investigate the impact of hyperglycemia on physiological processes, such as constitutive and injury-induced neurogenesis. The work also highlights the use of zebrafish to follow radiolabeled molecules (here, [18F]-FDG) using PET/CT.
FAQs
Researchers create Zebrafish Diabetes Model systems either by altering genes or by exposing animals to diabetogenic compounds. These approaches perturb glucose regulation and beta-cell function through different experimental routes, allowing investigators to examine whether observed changes arise from defined genetic alterations or induced disease-like stress. Comparing the routes helps align model design with the mechanism under study.
Measurements of blood glucose, insulin signaling, pancreatic development, and beta-cell regeneration provide complementary readouts. Glucose indicates systemic regulation, while insulin signaling and beta-cell observations connect that physiology to cellular function and tissue changes. Using these endpoints together helps researchers distinguish altered glucose control from defects in pancreatic development or reduced capacity for pancreatic repair.
Because zebrafish are transparent, researchers can examine pancreatic development and beta-cell regeneration in intact animals rather than relying only on isolated cells. This optical access helps connect cellular and molecular findings with whole-organism physiology. As a result, investigators can study how tissue-level changes relate to glucose regulation and disease progression within a living vertebrate system.
A typical study first generates the disease model through genetic manipulation or exposure to a diabetogenic compound. Researchers then monitor blood glucose, insulin signaling, pancreatic development, and beta-cell regeneration in the animals. Embryos and larvae can be maintained in multiwell plates, supporting organized observation of disease-related changes and responses to candidate therapies.
Rapid development, optical accessibility, and the ability to study embryos and larvae in multiwell plates make these systems practical for analyzing candidate therapies. Investigators can examine treatment-associated changes in glucose regulation, insulin signaling, pancreatic biology, or beta-cell regeneration. This format supports efficient comparison of responses while retaining whole-organism physiological context.
These models support studies of diabetes progression, pancreatic development, beta-cell function, and pancreatic repair. They also allow researchers to connect molecular and cellular mechanisms with changes occurring across an intact animal. In biology research, that combination is useful for investigating disease mechanisms and evaluating whether candidate therapies influence glucose regulation or regenerative processes.