Intestinal Resection Protocol
Intestinal Resection Protocol - Related Videos
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
03:58
Brain Tumor Resection in a Mouse Using a Minimally Invasive Resection System
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2025
Source: Alomari, S., et al. Implementation of Minimally Invasive Brain Tumor Resection in Rodents for High Viability Tissue Collection. J. Vis. Exp. (2022)This video demonstrates the use of a minimally invasive resection system (MIRS) to excise tumor tissue from an anesthetized mouse implanted with a tumor. The procedure involves securing the mouse onto a stereotactic frame, reopening the previous surgical site to access the brain, and inserting a specialized cannula that utilizes suction and a...
07:55
The Influence of Liver Resection on Intrahepatic Tumor Growth
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Cited by 6 •
2016
A high incidence of tumor recurrence after resection of liver metastases remains an unsolved problem. The illustrated mouse model may be useful to investigate the reasons for such recurrences. It combines a liver resection model with intrahepatic tumor cell injection for the first time.
06:55
Ileectomy-induced Bile Overaccumulation in Mouse Intestine
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Cited by 4 •
2017
Small intestine-dependent bile acid reabsorption and feedback inhibition of hepatic bile acid synthesis is important for systemic homeostasis and health. In this study, we describe a mouse model for ileal resection to evaluate ileectomy-induced bile malabsorption, overaccumulation, and toxicity in mouse intestine.
11:53
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
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Cited by 17 •
2017
This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this method.
FAQs
Tissue viability supports the integrity of the reconnected intestinal ends and helps preserve the conditions needed for postoperative healing. If viability is not maintained, recovery and gastrointestinal function may become difficult to interpret. Careful handling therefore strengthens experimental consistency and helps distinguish effects related to the cancer model or treatment from outcomes caused by compromised surgical tissue.
The anastomosis reconnects the remaining intestinal ends after the target segment has been removed. This step restores gastrointestinal continuity, allowing researchers to evaluate healing and intestinal function after surgery rather than studying an isolated excised segment. Consistent reconstruction also provides a comparable anatomical framework for assessing recovery, local recurrence, metastatic progression, or treatment response.
Standardizing the selected segment, excision process, reconnection, and postoperative monitoring reduces avoidable variation between experimental groups. Greater procedural consistency improves reproducibility and makes outcome differences easier to interpret. In cancer research, this is particularly important when investigators compare healing, intestinal function, recurrence, metastatic progression, or responses to treatment across otherwise similar studies.
The protocol proceeds through anesthesia, isolation of the target intestinal segment, excision of that segment, and reconnection of the remaining ends through an anastomosis. Researchers then monitor postoperative recovery. Keeping these stages consistent creates a controlled surgical model in which subsequent observations can be related to intestinal healing, function, or cancer-associated outcomes.
Postoperative assessment can focus on recovery, intestinal function, and healing of the reconstructed intestine. In cancer-focused experiments, researchers may also examine local recurrence, metastatic progression, or responses to treatment. Monitoring these outcomes connects the immediate surgical intervention with later biological effects and helps determine whether the model addresses the intended research question.
This approach is useful when a study needs to examine tumor-associated surgery within a controlled experimental setting. It can support investigations of how resection affects healing and intestinal function, while also providing a framework for studying local recurrence, metastatic progression, or treatment responses. Consistent technique enables more reliable comparisons among cancer research conditions and interventions.