Wavelength Selector
Wavelength Selector - Related Videos
02:32
The de Broglie Wavelength
0 Views •
2020
In the macroscopic world, objects that are large enough to be seen by the naked eye follow the rules of classical physics. A billiard ball moving on a table will behave like a particle; it will continue traveling in a straight line unless it collides with another ball, or it is acted on by some other force, such as friction. The ball has a well-defined position and velocity or well-defined momentum, p = mv, which is defined by mass m and velocity v at any given moment. This is the typical...
01:33
Synergistic Photobiomodulation with a Dual-Wavelength Laser in an In Vitro Disease Model
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2025
Divide the transwell system into five groups that undergo various treatments.The first group serves as the control and consists of a co-culture of neuroblastoma and microglia cells.In the second group, treat the co-culturer with fibrillar β-amyloid (fAβ) alone. fAβ induces damage in neuroblastoma cells.In the third group, treat neuroblastoma cells with an 808-nanometer laser, then co-culture them with untreated microglia.The laser reduces oxidative stress, improving neuronal survival.In the...
08:41
Single Wavelength Shadow Imaging of Caenorhabditis elegans Locomotion Including Force Estimates
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Cited by 2 •
2014
The technique presented here measures the path of freely swimming microscopic species using single wavelength exposure. C. elegans are used to demonstrate shadow imaging as an inexpensive alternative to costly microscopes. This technique can be adapted to accommodate various orientations, environments and species to measure direction, speed, acceleration and forces.
07:44
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Cited by 5 •
2016
Photostable cyanine dyes are attached to oligonucleotides to monitor hybridization by energy transfer.
10:32
Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
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Cited by 2 •
2021
Here, we present a protocol for cryogenic sample preparation and transfer of crystals into the vacuum endstation on beamline I23 at Diamond Light Source, for long-wavelength macromolecular X-ray crystallography experiments.
FAQs
Both components spread the light from a broad source according to wavelength, but they do so through different optical principles. A prism separates wavelengths as light passes through its material, whereas a diffraction grating separates them through its structured surface. The separated spectrum allows the instrument to position a selected region at the exit slit for chemical measurement.
Adjustable slits control which portion of the separated spectrum reaches the detector and determine the spectral bandwidth of the measurement. Narrowing the transmitted band can improve selectivity by reducing contributions from nearby wavelengths, while the selected band must still provide measurable intensity. This control is especially important when spectral features overlap in a chemical sample.
Optical filters offer a simpler approach than a monochromator because they isolate a selected wavelength range without requiring prism or diffraction-grating dispersion with adjustable slits. They can therefore support straightforward selective measurements when elaborate wavelength scanning is unnecessary. A monochromator is more suitable when the analysis requires controlled selection across different wavelength regions.
Selecting an appropriate wavelength can reduce spectral overlap between the analyte and other absorbing or emitting substances. That improved selectivity helps the measured intensity reflect the target chemical more reliably and can enhance sensitivity. Consequently, wavelength choice influences both identification and quantification, particularly in samples whose spectral signals occupy nearby regions.
In UV-visible absorption work, the selector is adjusted so the sample is measured with a chosen wavelength band from the broader source. The resulting intensity is then used to assess the sample's absorption and relate that measurement to analyte concentration. Selecting an appropriate band helps limit unrelated spectral contributions and supports more reliable chemical quantification.
Fluorescence measurements also depend on isolating an appropriate wavelength range from the available light. The selector helps focus the measurement on a chosen spectral region, improving the ability to distinguish the relevant fluorescence signal from other wavelengths. This selective measurement supports chemical identification and can contribute to concentration-related analysis when fluorescence intensity is used.