Cd31 Cd45
Cd31 Cd45 - Related Videos
05:32
Purification of CD31+ Endothelial Precursor Cells Using Magnetic-Activated Cell Sorting
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2025
This video demonstrates a technique for purifying CD31+ endothelial progenitor cells (EPCs) from a culture using magnetic-activated cell sorting (MACS). The cell suspension is treated with a fluorophore-conjugated antibody that binds to CD31 on the EPCs. Completing the labeling of CD31+ EPCs involves adding an antibody complex, which binds to the fluorophores, and a polymer-coated magnetic nanoparticle, which binds to the antibody complex. The labeled cells are then purified using a magnetic...
16:55
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
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Cited by 13 •
2011
In this article we demonstrate the isolation of murine resident lung mesenchymal stem cells (lung MSC), their expansion, characterization and analysis of immunomodulatory properties.
10:28
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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Cited by 8 •
2018
The differentiation of white and beige adipocytes from adipose tissue vascular progenitors bears potential for metabolic improvement in obesity. We describe protocols for a CD34+CD31+ endothelial cell isolation from human fat and for a subsequent in vitro expansion and differentiation into white and beige adipocytes. Several downstream applications are discussed.
08:52
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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Cited by 3 •
2017
Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.
08:56
Isolation of Murine Embryonic Hemogenic Endothelial Cells
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Cited by 11 •
2016
Hematopoietic stem and progenitor cells (HSPC) derive from specialized (hemogenic) endothelial cells during development, yet little is known about the process by which some endothelial cells specify to become blood forming. We demonstrate a flow-cytometry based method allowing simultaneous isolation of hemogenic endothelial cells and HSPC from murine embryonic tissues.
FAQs
Because their expression patterns overlap only partly, the pair provides a comparative profile rather than a single identity call. CD31 highlights endothelial-associated cells but can also appear on some blood cells, while CD45 marks hematopoietic cells. Examining both signals therefore helps distinguish endothelial, hematopoietic, and intermediate or transitional populations more effectively.
During embryonic blood development, researchers can compare cells according to their combined CD31 and CD45 signals to follow populations associated with vascular and hematopoietic states. This is especially useful for examining the endothelial-to-hematopoietic transition, where transitional populations are of interest. The profile helps organize candidate populations for lineage analysis without relying on either marker in isolation.
CD31 is enriched on endothelial cells, but it is also present on some blood cells. Consequently, CD31-positive cells cannot automatically be assigned an endothelial identity. Adding CD45 supplies complementary information about hematopoietic association, reducing ambiguity when researchers analyze developing vascular and blood populations during embryonic development.
Researchers label cells with fluorescent antibodies against CD31 and CD45, then examine the resulting signals by flow cytometry or microscopy. The two-marker pattern is used to characterize endothelial, hematopoietic, or transitional populations. Depending on the experimental design, identified populations can support cell isolation or subsequent lineage-analysis studies in developmental samples.
By assigning cells to marker-defined populations, the analysis supports questions about which cells are associated with endothelial or hematopoietic compartments and which may occupy a transitional category. Those assignments can guide cell isolation and lineage analysis, helping investigators connect marker patterns with the development of vascular and blood systems.
The approach is suited to studies that connect vascular development with blood formation. In embryonic samples, researchers can use the marker combination to characterize populations, examine the endothelial-to-hematopoietic transition, and select cells for lineage analysis. These applications make the pair useful for investigating how endothelial and hematopoietic systems emerge and relate to one another.