Mesodermal Cells
Mesodermal Cells - Related Videos
10:41
Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
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Cited by 8 •
2014
Somitogenesis is a rhythmic developmental process that spatially patterns the body axis of vertebrate embryos. Previously, we developed transgenic zebrafish lines that use fluorescent reporters to observe the cyclic genes that drive this process. Here, we culture dispersed cells from these lines and image their oscillations over time in vitro.
12:48
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
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Cited by 26 •
2012
Isolation of embryonic neural crest from the neural tube facilitates the use of in vitro methods for studying migration, self-renewal, and multipotency of neural crest.
04:19
Deriving Human Kidney Podocytes from Induced Pluripotent Stem Cells: A Procedure for Directed Differentiation of Mature Kidney Podocytes from Stem Cells
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2023
In this video, we describe the protocol to derive human kidney podocytes from induced pluripotent stem cells through cell differentiation under defined chemical conditions. The generated podocytes can be used for nephrotoxicity testing and disease modeling.
03:40
Metastatic Cancer Cell Colony Isolation from Chicken CAM: A Procedure to Isolate Cancer Cells from Shell-less Egg Cultures
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2023
In this video, we describe the isolation of metastatic cancer cell colonies from the chicken chorioallantoic membrane (CAM) using shell-less egg culture. This method helps screen for factors that may be responsible for cancer metastasis.
11:53
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
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Cited by 6 •
2016
To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.
FAQs
During gastrulation, mesodermal cells undergo coordinated movements that place them between the ectoderm and endoderm. This spatial arrangement is not merely structural: it establishes the tissue relationships needed for later patterning and organ formation. Consequently, changes in cell positioning can alter which developmental signals cells encounter, influencing their regional identity and subsequent specialization.
Signaling pathways and transcription factors work together to direct mesodermal development. Signals provide positional information, while transcription factors help establish and maintain gene-expression programs associated with particular regional identities. Their combined activity links an embryo’s spatial organization to cell-fate decisions, allowing developing populations to produce distinct muscle, connective, circulatory, skeletal, or urogenital derivatives.
Regional identity determines how mesodermal cells respond to developmental instructions and which specialized tissues they can produce. Cells with a common mesodermal origin therefore do not follow a single developmental path. Establishing these regional differences is essential for organized tissue patterning and organ formation, because differentiation must occur in the correct location and developmental context.
Researchers study cultured mesodermal progenitors and stem-cell-derived counterparts to examine developmental mechanisms outside the embryo. These models provide cellular systems for investigating how mesodermal populations acquire identity and develop specialized characteristics. They also connect fundamental developmental biology with studies of cardiovascular disease, musculoskeletal regeneration, and the potential use of cell-based therapies.
Cultured mesodermal progenitors and stem-cell-derived counterparts support several research areas. Investigators use them to study cardiovascular disease, explore musculoskeletal regeneration, examine developmental mechanisms, and assess the potential of cell-based therapies. Their broad usefulness comes from linking controlled cellular systems with questions about tissue formation, disease processes, and possible approaches to tissue repair.
Studying mesodermal cells helps researchers connect disrupted signaling, regional identity, or differentiation with abnormal tissue and organ formation. Because mesoderm contributes to several major body systems, developmental errors involving these cells can provide insight into congenital disorders affecting those systems. This perspective also clarifies how normal patterning and organ formation should proceed during development.