Live-line Tapping

Live-line tapping is an engineering method for connecting to a pressurized, operating pipeline or utility system without interrupting service. The process typically involves attaching a reinforced fitting and isolation valve to the line, pressure-testing the connection, and advancing a specialized cutter through the open valve to remove a section of the pipe wall; the valve then controls access while the new branch is placed in service. This technique supports pipeline modification, instrumentation installation, flow diversion, and emergency repairs while reducing downtime. Careful assessment of pressure, material compatibility, operating conditions, and containment is essential for safe execution.

Live-line Tapping - Related Videos

Research

JoVE Journal - Biology
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A Tactile Automated Passive-Finger Stimulator (TAPS)

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Cited by 24 •

2009

We describe a computer-controlled device for investigating the sense of touch: the Tactile Automated Passive-finger Stimulator (TAPS). We describe the components of TAPS, and show how TAPS is used to administer a two-interval forced-choice tactile grating orientation test.

Research

JoVE Journal - Biology

Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)

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Cited by 9 •

2013

Drosophila is famous for its powerful genetic manipulation, but not for its suitability of in-depth biochemical analysis. Here we present a TAP-based procedure to identify interacting partners of any protein of interest from the fly brain. This procedure can potentially lead to new avenues of research.

Research

JoVE Journal - Biochemistry
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Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

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Cited by 1 •

2018

We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

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Cited by 27 •

2017

This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.

Uncovering Beat Deafness: Detecting Rhythm Disorders with Synchronized Finger Tapping and Perceptual Timing Tasks

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Cited by 35 •

2015

Behavioral tasks that allow for the assessment of perceptual and sensorimotor timing abilities in the general population (i.e., non-musicians) are presented. Synchronization of finger tapping to the beat of an auditory stimuli and detecting rhythmic irregularities provides a means of uncovering rhythm disorders.

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