Primary T Lymphocytes
Primary T Lymphocytes - Related Videos
08:39
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
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Cited by 27 •
2010
T lymphocyte migration occurs during homing to lymphoid organs, exit from the vasculature, and entering into peripheral tissues. Here, we describe a protocol that can be used to analyze T lymphocyte migration in vitro.
09:14
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
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Cited by 21 •
2014
Static adhesion assay is a powerful tool that can be used to model the interactions between T lymphocytes and other cell types. Interactions are generated by injecting labeled T cells into wells coated with adhesion molecules, while a plate reader is used to quantify the number of adherent cells following serial washes.
12:59
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
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Cited by 33 •
2013
With effector functions distinct from other T cell subsets, Th17 cells have been centrally implicated in inflammatory autoimmunity. This in vitro Th17 differentiation protocol provides a means to determine whether naïve CD4+ T lymphocytes can differentiate into Th17 cells, and to further examine their role in autoimmunity and host response.
06:02
Isolation of Lymphocytes from a Mouse Peyer's Patch
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2025
In this video, we demonstrate the isolation of lymphocytes from a harvested mouse Peyer's patch. The isolated lymphocytes can be used for further analysis.
08:08
Isolation and Activation of Murine Lymphocytes
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Cited by 56 •
2016
Lymphocytes are the major players in adaptive immune responses. Here, we present a lymphocyte purification protocol to determine the physiological functions of the desired molecules in lymphocyte activation in vitro and in vivo. The described experimental procedures are suitable for comparing functional capacities between control and genetically modified lymphocytes.
FAQs
Antigen recognition begins when T-cell receptors detect peptide antigens displayed by major histocompatibility complex molecules. Co-stimulatory signals then help determine whether activation proceeds, while cytokines influence clonal expansion, differentiation, or cytotoxic activity. Examining these interacting inputs allows researchers to connect genetic changes with specific stages of immune-cell behavior rather than measuring activation as a single outcome.
Primary T lymphocytes retain donor-specific characteristics that may be changed or lost in immortalized cell lines. Those characteristics provide a more physiologically relevant setting for comparing inherited immune traits, gene expression, and signaling responses. Consequently, researchers can investigate whether a genetic variant produces different cellular effects in cells that preserve features of the originating organism.
Researchers can examine how a variant influences gene expression, signaling pathways, activation, clonal expansion, differentiation, or cytotoxic activity in primary T lymphocytes. Comparing these cellular outcomes with the relevant genetic background helps move beyond identifying an association and toward understanding biological function. This approach is particularly useful when variants are suspected of altering immune regulation.
Gene-expression and signaling analyses can reveal how genetic factors regulate T-cell responses after antigen recognition and supporting immune signals. They may distinguish effects on activation from effects on expansion, differentiation, or cytotoxic function. In genetics research, this separation helps identify which cellular pathway is associated with an inherited immune trait or disease-related mechanism.
Because these cells are antigen-responsive and retain donor-specific biology, they can be used to evaluate the functional consequences of targeted genetic modification. Investigators can compare cellular responses before and after modifying a gene, focusing on immune regulation and downstream behavior. The resulting comparison supports assessment of whether a genetic change produces the intended biological effect in a relevant cell system.
They are relevant when researchers need to connect genetic variation or gene manipulation with immune-cell function. Their use supports investigation of disease mechanisms, evaluation of gene therapies, and assessment of functional effects from genetic variants. Findings can be interpreted through outcomes such as altered activation, expansion, differentiation, or cytotoxic activity, while retaining donor-specific context.