Mouse Cremaster
Mouse Cremaster - Related Videos
07:19
The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation
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Cited by 46 •
2011
A tissue preparation is described for visualization and experimental manipulation of the living microcirculation. In anesthetized male mice, the thin, highly vascularized cremaster muscle is prepared for intravital microscopy to study microvascular networks including arterioles, capillaries and venules. This preparation is readily adapted for rats and hamsters.
07:36
Intravital Microscopy of the Microcirculation in the Mouse Cremaster Muscle for the Analysis of Peripheral Stem Cell Migration
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Cited by 4 •
2013
Intravital microscopy of the mouse M. cremaster microcirculation offers a unique and well-standardized in vivo model for the analysis of peripheral bone marrow stem cell migration.
06:10
Visualization and Quantification of the Cell-free Layer in Arterioles of the Rat Cremaster Muscle
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Cited by 1 •
2016
This study demonstrates the surgical preparation of the rat cremaster muscle for the visualization of the in vivo cell-free layer. Considerable factors affecting the accuracy of the cell-free layer width measurement are discussed in this study.
15:07
Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle
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Cited by 10 •
2012
Digital intravital epifluorescence microscopy of postcapillary venules in the cremasteric microcirculation is a convenient method to gain insights into leukocyte-endothelial interaction in vivo in ischemia-reperfusion injury (IRI) of striated muscle tissue. We here provide a detailed protocol to safely perform the technique and discuss its applications and limitations.
08:11
Leukocyte Infiltration of Cremaster Muscle in Mice Assessed by Intravital Microscopy
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Cited by 7 •
2020
Here, we show how to perform intravital microscopy on post-capillary venules of the mouse cremaster muscle. Commonly applied to different models of inflammation and sepsis, particularly those induced by chemokines and cytokines, we highlight its relevance in the study of muscolopathies involving exaggerated muscular leukocyte infiltration.
FAQs
These behaviors represent successive stages of leukocyte recruitment through venules. Rolling and adhesion indicate interactions with an activated vascular lining, whereas crawling and transmigration show progression toward tissue entry. Intravital microscopy allows researchers to observe and quantify these stages directly, helping distinguish whether an inflammatory condition or treatment alters initial capture, stable attachment, or passage across the vessel wall.
Endothelial activation changes the local vascular environment in ways that influence leukocyte recruitment, while chemokine signaling helps guide immune cells through the recruitment sequence. Applying inflammatory stimuli or infectious agents allows these processes to be examined over time. The resulting observations connect vascular responses with immune-cell behavior rather than measuring either process in isolation.
The preparation enables simultaneous investigation of cellular recruitment and changes in the vessel environment during inflammation or infection. Researchers can relate leukocyte rolling, adhesion, crawling, and transmigration to vascular permeability, producing a broader picture of inflammatory function. This relationship is useful for determining whether altered immune-cell trafficking accompanies changes in microvascular barrier behavior.
Preserving local circulation maintains the in vivo relationship between the microvasculature, endothelium, and circulating immune cells while the tissue is observed. Superfusion supports direct microscopic examination without losing access to the local vascular environment. Consequently, researchers can assess dynamic recruitment and vascular responses under conditions that retain physiological connections relevant to inflammation and infection.
Researchers exteriorize the cremaster tissue, maintain it by superfusion while local circulation remains intact, and use intravital microscopy to observe the microvasculature. They then apply an inflammatory stimulus or infectious agent and follow leukocyte behavior and vascular responses over time. This workflow produces visual and quantitative information about recruitment, endothelial activation, and permeability.
The preparation is useful when investigators need to examine how an infectious agent changes host vascular and immune responses in vivo. Microscopy can reveal effects on leukocyte recruitment, endothelial activation, chemokine signaling, and vascular permeability over time. These measurements help characterize host-pathogen interactions and identify how infection modifies the sequence of immune-cell movement through venules.
An intervention can be assessed by comparing its effects on the observed inflammatory and infectious responses. Relevant outcomes include changes in leukocyte rolling, adhesion, crawling, transmigration, endothelial activation, chemokine-associated recruitment, or vascular permeability. Because the preparation supports direct, quantitative observation in vivo, it can show whether treatment modifies immune-cell behavior or microvascular function.