Laser Doppler Flowmetry
Laser Doppler Flowmetry - Related Videos
07:26
Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat
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Cited by 26 •
2013
Several animal models of cerebral ischemia have been developed to simulate the human condition of stroke. This protocol describes the endothelin-1 (ET-1) induced middle cerebral artery occlusion (MCAO) model for ischemic stroke in rats. In addition, important considerations, advantages, and shortcomings of this model are discussed.
07:12
Evaluation of Cerebral Blood Flow Autoregulation in the Rat Using Laser Doppler Flowmetry
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Cited by 4 •
2020
This article demonstrates the use of laser Doppler flowmetry to evaluate the ability of the cerebral circulation to autoregulate its blood flow during reductions in arterial blood pressure.
13:01
Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice
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Cited by 76 •
2011
The intraluminal middle cerebral artery occlusion (MCAO) model is the most frequent used model among experimental ischemic stroke models. Here we will demonstrate the entire model in detail with the guide of Laser Doppler flowmetry, and its representative results.
09:28
Using Laser Doppler Imaging and Monitoring to Analyze Spinal Cord Microcirculation in Rat
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Cited by 5 •
2018
Here we present a combination of laser Doppler perfusion imaging (LDPI) and laser Doppler perfusion monitoring (LDPM) to measure spinal cord local blood flows and oxygen saturation (SO2), as well as a standardized procedure for introducing spinal cord trauma on rat.
10:39
Laser Doppler: A Tool for Measuring Pancreatic Islet Microvascular Vasomotion In Vivo
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Cited by 7 •
2018
Pancreatic islet microvascular vasomotion regulates islet blood distribution and maintains the physiological function of islet β cells. This protocol describes using a laser Doppler monitor to determine the functional status of pancreatic islet microvascular vasomotion in vivo and to assess the contributions of pancreatic islet microcirculation to pancreatic-related diseases.
FAQs
The measured signal combines two biologically meaningful variables: the number of moving red blood cells and their velocity. Motion changes the frequency of reflected laser light, producing Doppler shifts that can be analyzed as a perfusion estimate. Consequently, a change in the recorded value may reflect altered cell movement, altered cell abundance, or both within the sampled tissue volume.
The sampled tissue volume determines which microvascular region contributes to the recorded signal. The resulting perfusion estimate therefore describes blood cell motion within that specific volume rather than automatically representing circulation throughout the entire organ or limb. Keeping this spatial context in mind helps researchers relate measurements to local tissue viability, peripheral circulation, or wound-related changes.
Continuous measurement allows blood-flow changes to be followed as they occur, rather than captured only at isolated time points. This real-time capability is useful when tissue perfusion changes during a physiological challenge or after a drug is administered. It also supports monitoring over time while leaving the examined tissue undisturbed, which is valuable in medicine and vascular research.
A low-power laser first illuminates the selected tissue. Reflected light is then collected and examined for frequency changes produced by moving red blood cells. The analyzed Doppler signal is converted into an estimate of local perfusion, reflecting cell number and velocity within the sampled volume. This workflow provides a continuous measurement without disrupting the tissue being assessed.
Laser Doppler Flowmetry can support assessment of tissue viability, peripheral circulation, and wound healing. Its noninvasive format permits repeated or ongoing observation of local perfusion, while the continuous signal can reveal changes during monitoring. These features make it relevant when investigators need information about microvascular blood flow without interrupting the tissue under study.
The technique records perfusion changes during or after a drug exposure or physiological challenge, allowing the response to be followed over time. Because the measurement is continuous, investigators can compare the evolving signal with the intervention or challenge rather than relying only on a single endpoint. In medicine, this helps characterize local vascular responses and their timing.