Tissue Engineered Vascular Graft
Tissue Engineered Vascular Graft - Related Videos
11:17
Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring
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Cited by 9 •
2015
A step-by-step protocol for the inter-positional placement of Tissue Engineered Vessels (TEVs) into the carotid artery of a sheep using end-to-end anastomosis and real-time digital assessment in vivo until animal sacrifice.
09:55
Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
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Cited by 17 •
2016
This is a guideline for constructing in vivo vascularized tissue using a microsurgical arteriovenous loop or a flow-through pedicle configuration inside a tissue engineering chamber. The vascularized tissues generated can be employed for organ regeneration and replacement of tissue defects, as well as for drug testing and disease modeling.
11:22
Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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Cited by 32 •
2011
Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.
13:04
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Cited by 13 •
2015
Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.
08:53
The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering
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Cited by 38 •
2016
We describe a microsurgical approach for the generation of an arteriovenous (AV) loop as a model for analyzing vascularization in vivo in an isolated and well-characterized environment. This model is not only useful for the investigation of angiogenesis, but is also optimally suited for engineering axially vascularized and transplantable tissues.
FAQs
Performance depends on coordinating scaffold degradation with new matrix formation. As the scaffold gradually breaks down, vascular cells must deposit extracellular matrix, the structural material that helps maintain vessel integrity. If these processes are not balanced, remodeling may not produce a durable conduit. This relationship makes degradation control a central design goal in graft research.
Endothelialization refers to establishing an endothelial cell lining on the graft’s blood-contacting surface. This process is studied because the interface between the graft and circulating blood affects how well the construct functions within the host circulation. Controlling endothelial cell behavior can therefore support blood-flow compatibility and improve integration during vascular remodeling.
Unlike conventional synthetic or donor-derived options, a tissue engineered vascular graft is investigated for its capacity to support tissue formation and remodeling after implantation. This potential is especially relevant when suitable vessels are unavailable or when graft failure is a concern. Research therefore emphasizes creating conduits that can integrate with the host rather than serving only as passive replacements.
Preparation typically combines a three-dimensional scaffold with one or more biological elements, including vascular cells, biomaterials, or signaling factors. The scaffold supplies an organized framework, while the added components are intended to influence cell behavior and tissue development. Researchers then examine whether the construct can support extracellular matrix deposition, mechanical strength, and compatibility with blood flow.
This approach may be relevant for cardiovascular and reconstructive procedures when an appropriate vessel is unavailable or when failure of an existing graft is a significant concern. Its medical promise comes from supporting tissue formation and host integration rather than relying solely on a permanent substitute. The technology remains an area of research focused on achieving durable vascular conduits.
Studies commonly evaluate scaffold degradation, vascular cell behavior, endothelialization, extracellular matrix deposition, mechanical strength, and integration with the host circulation. Together, these outcomes indicate whether conditioning has produced a construct capable of remodeling while maintaining structural function. The findings help researchers assess durability and determine whether a graft is suitable for cardiovascular or reconstructive applications.