Cell Attached Recording
Cell Attached Recording - Related Videos
13:07
One-channel Cell-attached Patch-clamp Recording
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Cited by 17 •
2014
Described here is a procedure for obtaining long stretches of current recording from one ion channel with the cell-attached patch-clamp technique. This method allows for observing, in real time, the pattern of open-close channel conformations that underlie the biological signal. These data inform about channel properties in undisturbed biological membranes.
01:20
Attachment
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2025
Attachment is vital for infant development, as warm social interactions support growth and well-being. In a classic 1958 study by Harry Harlow, the significance of warmth and comfort in forming attachments was examined. Harlow separated newborn monkeys from their mothers and provided two artificial "mothers": one made of cold wire and the other covered in soft cloth. Despite the wire mother offering food, the infant monkeys preferred the comfort of the cloth mother, demonstrating that physical...
08:34
Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
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Cited by 1 •
2016
The first step in the arenavirus life cycle is attachment of viral particles to host cells. We report a quantitative (q)RT-PCR-based assay for ultrasensitive detection and quantitation of arenavirus attachment events.
03:34
An In Vitro Assay to Study Arenavirus Attachment to the Host Cell
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2026
Source: Klaus, J. P., et al Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment. J. Vis. Exp. (2016).This video demonstrates how to measure arenavirus attachment to monkey kidney cells. The assay uses low temperatures to block endocytosis and isolate the virus binding step. The approach enables precise quantification of surface-bound virus particles under conditions that restrict internalization.
10:31
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
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Cited by 30 •
2012
Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
FAQs
The seal electrically isolates the membrane patch beneath the glass micropipette, so currents through ion channels in that patch can be detected with limited interference from the surrounding cell. Seal quality therefore influences how clearly channel events or electrical activity appear. A stable seal supports measurements that preserve the cell's largely undisturbed state and approximate near-physiological signaling conditions.
Because the membrane is not ruptured, the neuron remains largely undisturbed during measurement. This preservation allows spontaneous firing and synaptic responses to be examined in a state closer to normal cellular function than would be possible after substantially disrupting the membrane. The resulting observations can better reflect how neuronal excitability and communication operate under near-physiological conditions.
The membrane patch beneath the pipette can reveal ion-channel currents as well as action-potential activity. These signals provide complementary information: channel currents describe activity at the membrane-patch level, whereas action-potential recordings indicate electrical events associated with neuronal firing. Examining both types helps connect individual membrane events with broader patterns of neuronal excitability.
Researchers can compare spontaneous firing, synaptic responses, and channel behavior across experimental conditions. Differences in these signals may indicate altered neuronal excitability or changes in electrical communication. This comparison is useful when testing how drugs, circuit conditions, or disease-related changes affect neurons, because the cell remains sufficiently intact to preserve relevant signaling behavior during measurement.
The setup requires positioning a fine glass micropipette against the cell membrane and forming a high-resistance seal. The membrane is not ruptured, allowing the pipette to monitor the patch directly beneath its tip. Once the seal is established, researchers record ion-channel currents or action-potential activity while maintaining the cell in a largely undisturbed condition.
This configuration is useful when the goal is to observe neuronal activity without substantially disturbing the recorded cell. It supports studies of spontaneous firing, synaptic responses, and membrane-channel behavior, making it relevant for characterizing neuronal excitability and identifying functional cell types. It can also reveal how pharmacological, circuit-related, or disease-associated conditions modify electrical communication.
Recordings can show whether a neuron fires spontaneously, how it responds to synaptic input, and how ion channels behave within the sampled membrane patch. Together, these observations help researchers characterize functional cell types and assess changes in excitability. In neuroscience experiments, the measurements can connect cellular electrical behavior with altered circuit or disease-related signaling.