Coast Down Test

A coast down test is an experimental method for measuring how a moving vehicle slows when its propulsion is removed, revealing the effects of resistive forces. During the test, a vehicle is brought to a specified speed and allowed to roll in neutral or with the drivetrain disengaged while speed and time are recorded. The resulting deceleration is analyzed under controlled conditions to separate contributions from rolling resistance, aerodynamic drag, and mechanical losses. In physics and automotive engineering, coast down data help estimate drag coefficients, validate vehicle models, compare design changes, and assess energy efficiency without relying on powered acceleration measurements.

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Research

JoVE Journal - Biology

Near-Infrared Spectroscopy During Reactive Hyperemia for the Assessment of Lower Limb Vascular Function

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Cited by 3 •

2024

Here, we describe a non-invasive approach using near-infrared spectroscopy to assess reactive hyperemia in the lower limb. This protocol provides a standardized assessment of vascular and microvascular responsiveness that may be used to determine the presence of vascular dysfunction as well as the efficacy of therapeutic interventions.

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

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Cited by 6 •

2014

MicroRNAs (miRNAs) are a widely conserved class of regulatory molecules. Here we describe a miRNA cloning method that relies upon two potent ligation steps followed by high-throughput sequencing. Our method permits accurate genome-wide quantitation of miRNAs.

Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools

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Cited by 10 •

2020

Here, we present a protocol to use a curvelet transform-based, open-source MATLAB software tool for quantifying fibrillar collagen organization in the extracellular matrix of both normal and diseased tissues. This tool can be applied to images with collagen fibers or other types of line-like structures.

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

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Cited by 2 •

2023

The protocol summarizes the best practices to minimize microbial bioburden in a cleanroom environment and includes strategies such as environmental monitoring, process monitoring, and product sterility testing. It is relevant for manufacturing and testing facilities that are required to meet current good tissue practice standards and current good manufacturing practice standards.

Research

JoVE Journal - Biochemistry
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Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

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Cited by 9 •

2019

Described is a proteomics workflow for identifying protein interaction partners from a nuclear subcellular fraction using immunoaffinity enrichment of a given protein of interest and label-free mass spectrometry. The workflow includes subcellular fractionation, immunoprecipitation, filter aided sample preparation, offline cleanup, mass spectrometry, and a downstream bioinformatics pipeline.

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