3d Epithelial Tissues
3d Epithelial Tissues - Related Videos
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
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Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
12:22
Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models
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Cited by 80 •
2012
A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate formation is described. The aggregates generated display in vivo-like characteristics not observed in conventional culture models and serve as a more accurate organotypic model system for a multitude of scientific investigations.
08:49
Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
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Cited by 6 •
2016
This manuscript describes a soft lithography-based technique to engineer uniform arrays of three-dimensional (3D) epithelial tissues of defined geometry surrounded by extracellular matrix. This method is amenable to a wide variety of cell types and experimental contexts and allows for high-throughput screening of identical replicates.
06:33
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
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Cited by 132 •
2015
We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.
01:23
Epithelial Tissues and Their Functions
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2025
Epithelial tissues are large sheets of cells covering all of the surfaces of the body. These surfaces can be internal or external, for example, skin, airways, the digestive tract, the urinary system, and the reproductive system. Hollow organs and body cavities that do not connect to the body's exterior, including blood vessels and serous membranes, are lined by epithelial tissue known as the endothelium. Epithelial tissues provide the body's first line of protection from physical, chemical, and...
FAQs
Apical-basal polarity gives epithelial cells an organized orientation, while cell-cell junctions connect neighboring cells into a coordinated layer. Together, these features help the tissue establish barrier behavior and regulate how substances move across or between cells. Their preservation is therefore important when using engineered models to study epithelial transport, secretion, or tissue responses.
The extracellular matrix provides a surrounding environment with which epithelial cells interact as they organize in three dimensions. These interactions contribute to the formation of spheroids, organoids, or layered tissues rather than isolated cellular arrangements. In bioengineering, incorporating matrix interactions helps models represent tissue organization and remodeling more realistically than a flat culture alone.
Three-dimensional organization allows epithelial cells to establish spatial relationships, barrier structures, and interactions with their surrounding matrix. As a result, models can represent transport, secretion, and tissue remodeling in a more tissue-like setting. This added organization is especially relevant when chemical or mechanical stimuli produce responses that depend on the structure of the epithelial layer.
Chemical and mechanical stimuli can be examined through the changes they produce in epithelial behavior and tissue organization. Because 3D models preserve polarity, junctions, and matrix interactions, they provide a setting for studying responses within an organized tissue rather than within dispersed or planar cells. This supports investigations of how epithelial structures adapt or remodel under experimental conditions.
Bioengineers should consider whether the model reproduces apical-basal polarity, cell-cell junctions, extracellular-matrix interactions, and relevant barrier functions. They should also match the structure, such as a spheroid, organoid, or layered tissue, to the question being studied. These characteristics determine whether the model can meaningfully represent transport, secretion, remodeling, or stimulus responses.
They are useful when toxicity needs to be evaluated in a tissue-like epithelial context rather than only in a two-dimensional cell layer. Their three-dimensional organization can support assessment of effects on barrier functions, transport, secretion, or remodeling. This makes them relevant platforms for investigating how chemical exposures influence epithelial tissues during biomedical and bioengineering research.
These models can guide the design of tissue-engineered grafts by showing how epithelial cells organize, establish junctions, interact with extracellular matrix, and maintain barrier-related functions. Their capacity to form layered tissues or other organized structures also supports regenerative-medicine research. Such information can help evaluate whether engineered constructs reproduce important features of epithelial tissue organization.