Albumin Reuptake
Albumin Reuptake - Related Videos
08:26
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2018
The protocols for studying the binding of gold cations (Au(III)) to various conformations of bovine serum albumin (BSA) as well as for characterizing the conformational dependent unique BSA-Au fluorescence are presented.
07:22
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Cited by 15 •
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In this study, we present an easy and efficient procedure for evaluating the disruption of the blood-brain barrier under neurodegenerative conditions. To achieve our objective, we infused high molecular weight fluorescein isothiocyanate labelled (FITC)-albumin into mouse jugular vein and evaluated its leakage into the brain parenchyma by fluorescence microscopy.
07:41
Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0
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Cited by 2 •
2017
Fast-scan cyclic voltammetry can monitor in vivo dopamine neurotransmission in the context of drugs, disease, and other experimental manipulations. This work describes the implementation of QNsim1.0, a software to model electrically stimulated dopamine responses according to the quantitative neurobiological model to quantify estimates of dopamine release and reuptake dynamics.
02:47
The Synapse
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2019
Neurons communicate with one another by passing on their electrical signals to other neurons. A synapse is the location where two neurons meet to exchange signals. At the synapse, the neuron that sends the signal is called the presynaptic cell, while the neuron that receives the message is called the postsynaptic cell. Note that most neurons can be both presynaptic and postsynaptic, as they both transmit and receive information. An electrical synapse is one type of synapse in which the pre-...
05:42
The Forced Swim Test as a Model of Depressive-like Behavior
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Cited by 686 •
2015
This protocol describes the forced swim test, which is used for the study of depressive-like behavior in rodents. This procedure involves placing an animal in a container filled with water that eventually will lead to the exhibition of immobility behavior, which is considered to reflect behavioral despair.
FAQs
The megalin-cubilin system provides the recognition step at the proximal tubule brush border. These receptors bind albumin in tubular fluid and initiate endocytosis, allowing epithelial cells to retrieve the protein rather than leave it in the tubular stream. In a bioengineered model, this receptor-dependent uptake is therefore a specific indicator of epithelial transport function.
Endosomal trafficking determines what happens after albumin enters the epithelial cell. The internalized protein is directed toward lysosomal degradation, while its components are returned to the cell. This sequence links surface binding to intracellular processing and recovery of usable material. Disruption at either the uptake or trafficking stage could therefore produce an abnormal reuptake readout, even if filtration is represented.
Albumin reuptake is informative because it connects a filtration event with downstream tubular handling. Healthy kidney function requires more than glomerular filtration alone; proximal tubule cells must also retrieve filtered albumin and process it through the endosomal pathway. Consequently, a model that captures this response can reveal tubular epithelial performance that would be missed by measuring filtration without assessing cellular uptake.
Kidney organoids, engineered renal tissue, and kidney-on-a-chip platforms can use this pathway to test whether proximal tubule-like epithelium performs protein transport. The readout adds a functional dimension to model characterization: it shows whether cells bind, internalize, and process filtered albumin, rather than merely representing renal tissue. This supports comparisons among bioengineered systems.
Measuring albumin retrieval provides a functional readout of epithelial transport in a model. Researchers can use the result to determine whether a kidney organoid, renal tissue construct, or chip reproduces proximal-tubule handling after filtration. A diminished or altered retrieval response can flag impaired tubular performance, making the pathway useful for model validation and comparison.
Proteinuria involves abnormal protein handling, so albumin reuptake offers a way to examine the tubular side of that problem after filtration. Studying receptor-mediated retrieval and intracellular processing in engineered renal models can show whether the epithelial transport pathway is functioning appropriately. This complements filtration-focused analyses by addressing what happens to albumin once it reaches tubular fluid.
Because albumin reuptake depends on receptor binding, endocytosis, and subsequent endosomal processing, it can serve as a functional indicator of proximal tubule activity. Engineered kidney systems can evaluate whether drug exposure disrupts this transport pathway. Changes in albumin handling may therefore help assess drug-related tubular injury alongside broader characterization of renal tissue models.