Laser Application
Laser Application - Related Videos
08:06
Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications
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Cited by 3 •
2017
Protocols for the synthesis of microspheres from polymers, the manipulation of microspheres, and micro-photoluminescence measurements are presented.
10:06
Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair
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Cited by 23 •
2012
Sutures are usually needed to repair tissue during surgical procedures. However, their application can be problematic as they are invasive and may damage tissue. The fabrication and application methods of a novel tissue adhesive are here reported. This adhesive film is laser-activated and does not require the use of sutures.
08:14
Measuring Spray Droplet Size from Agricultural Nozzles Using Laser Diffraction
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Cited by 30 •
2016
We present protocols to be used in the measurement of spray droplet size from agricultural nozzles used in both aerial and ground based agrochemical applications. These methods presented were developed to provide consistent and repeatable droplet size data both inter- and intra-laboratory, when using laser diffraction systems.
08:57
Laser Microdissection for Species-Agnostic Single-Tissue Applications
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Cited by 5 •
2022
A protocol is described that uses laser microdissection to isolate individual nematode tissues for RNA-sequencing. The protocol does not require species-specific genetic toolkits, allowing gene expression profiles to be compared between different species at the level of single-tissue samples.
08:18
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
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Cited by 30 •
2017
Confocal fluorescence microscopy and laser micro-irradiation offer tools for inducing DNA damage and monitoring the response of DNA repair proteins in selected sub-nuclear areas. This technique has significantly advanced our knowledge of damage detection, signaling, and recruitment. This manuscript demonstrates these technologies to examine single and double strand break repair.
FAQs
These parameters determine how energy interacts with a target and how much surrounding material is exposed. Wavelength selection helps match the light to the intended biological or engineered material, while intensity controls energy delivery. Pulse duration influences whether the dominant result is photothermal, photochemical, or mechanical, allowing researchers to tailor processing, measurement, or manipulation.
The three effect categories provide different ways to influence biological materials and engineered systems. Photothermal effects use light-generated heat, photochemical effects drive light-dependent chemical changes, and mechanical effects produce physical action. Selecting among them helps researchers align the laser process with goals such as tissue processing, cell manipulation, microfabrication, or light-based therapy.
Focusing concentrates energy on a selected target rather than distributing it broadly across the sample. Combined with appropriate wavelength, intensity, and pulse duration, this approach can produce a localized response while limiting exposure to nearby regions. That spatial control supports high-resolution microscopy, targeted tissue processing, and fabrication of small-scale biomaterials or microdevices.
The intended outcome depends on how the optical parameters interact with the target and its surrounding environment. Researchers adjust wavelength, intensity, and pulse duration to favor measurement, material modification, or physical control. These choices also affect the balance between the desired response and unwanted exposure, which is central to reliable biological analysis and engineered-system processing.
A typical workflow begins by identifying the target and desired outcome, then selecting wavelength, intensity, and pulse duration to produce the relevant interaction. The beam is focused or directed toward the selected region, and the resulting measurement, modification, or control is assessed. This parameter-based workflow can be adapted for microscopy, tissue processing, fabrication, or therapy development.
Laser application supports several bioengineering activities, including high-resolution microscopy, tissue processing, microfabrication, cell manipulation, and light-based therapies. It also contributes to biomaterial and microdevice construction, where localized energy can help create or modify engineered structures. These uses extend across biological analysis, device development, diagnosis, treatment, and regenerative medicine.
In construction-oriented bioengineering, focused laser energy can modify materials with localized control, supporting the creation of biomaterials and microdevices. Researchers select optical conditions according to the desired interaction with the material and the required spatial resolution. The resulting precision is useful when engineered structures must be produced or processed without broadly affecting adjacent regions.
Laser methods can deliver controlled optical effects to selected biological regions, supporting precise analysis, treatment development, and tissue-related processing. Their ability to limit exposure beyond the target is relevant to minimally invasive approaches. In regenerative medicine, the same control contributes to biomaterial construction and engineered systems intended to support biological repair or intervention.