Perfusion Decellularization
Perfusion Decellularization - Related Videos
11:30
Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
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Cited by 68 •
2012
A method to rapidly and completely remove cellular components from an intact porcine heart through retrograde perfusion is described. This method yields a site specific cardiac extracellular matrix scaffold which has the potential for use in multiple clinical applications.
09:38
Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device
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Cited by 18 •
2015
The presented techniques for liver harvesting, cannulation and perfusion using our proprietary device enable sophisticated perfusion set-ups to improve decellularization and recellularization experiments in rat livers.
09:24
Decellularization and Recellularization of Whole Livers
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Cited by 39 •
2011
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
05:17
Cardiopulmonary Complex Decellularization: A Technique for Decellularizing Heart and Lungs from Murine Model
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2025
This video demonstrates a method of producing decellularized extracellular matrix or ECM scaffolds by applying antegrade perfusion of detergents through the vasculature. The structural ECM scaffolds can then be used to analyze ECM remodeling associated with cardiopulmonary disease.
10:56
Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
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Cited by 4 •
2022
The protocol describes the surgical procurement and subsequent decellularization of vascularized porcine flaps by the perfusion of sodium dodecyl sulfate detergent through the flap vasculature in a customized perfusion bioreactor.
FAQs
Detergents, enzymes, and washing solutions act through complementary mechanisms during perfusion decellularization. Circulation through the tissue’s vascular network delivers these agents throughout the organ, enabling chemical and mechanically assisted disruption of cellular membranes and nucleic acids. Because exposure is controlled, the process can remove cellular material while retaining key extracellular matrix components such as collagen and elastin. This balance supports later study or engineering.
The vascular network provides the internal pathway that makes perfusion decellularization possible. Solutions can be circulated through existing channels rather than applied only to the outer surface, exposing internal regions to detergents, enzymes, and washes. This route links chemical delivery with preservation of vascular architecture, allowing the remaining scaffold to retain organ-scale geometry and a biologically relevant structural framework.
Collagen and elastin are important preservation targets because they are structural components of the extracellular matrix. Retaining them, together with the tissue’s three-dimensional geometry, preserves more than shape: the scaffold can also retain tissue-specific biochemical cues. These features make the decellularized material useful for studying matrix biology and for designing engineered grafts that reflect the original tissue context.
The workflow combines detergents, enzymes, and washing solutions circulated through the tissue’s vascular network. Chemical treatments disrupt cellular membranes and nucleic acids, while circulation supplies the process’s mechanical component. Washing helps remove disrupted cellular material. The desired outcome is an acellular scaffold whose extracellular matrix and vascular architecture remain sufficiently preserved for subsequent study, engineering, or recellularization research.
Researchers can use the resulting scaffold to study matrix biology in a three-dimensional, tissue-specific setting. Its preserved geometry and biochemical cues also support bioengineering efforts aimed at developing engineered grafts. This approach provides structural information alongside extracellular matrix features, making it relevant when investigators need to examine tissue organization within a preserved organ or tissue framework.
After decellularization, recellularization introduces appropriate cells into the preserved scaffold as a separate research step. The goal is to explore whether retained architecture and matrix cues can support more physiologically relevant tissues. In bioengineering, this strategy informs regenerative medicine and transplantation research by connecting scaffold preservation with efforts to develop tissues that better reflect native structural conditions.