Cell Wall Glycans

Cell wall glycans are carbohydrate chains and polymers that form much of the structural framework surrounding cells in plants, fungi, algae, and many bacteria. Their monosaccharide composition, glycosidic linkages, branching, and interactions with proteins or other polymers determine wall architecture, strength, porosity, and flexibility. In plant cell walls, cellulose microfibrils are embedded in hemicellulose and pectin networks, while fungal walls commonly contain β-glucans and chitin; bacterial glycans contribute to peptidoglycan and extracellular matrices. Studying these materials helps explain cell growth, development, microbial survival, host-pathogen interactions, and responses to environmental stress, with applications in biotechnology, agriculture, medicine, and biomaterials research.

Cell Wall Glycans - Related Videos

Research

JoVE Journal - Biology

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

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Cited by 26 •

2012

A technique called Comprehensive Microarray Polymer Profiling (CoMPP) for the characterisation of plant cell wall glycans is described. This method combines the specificity of monoclonal antibodies directed to defined glycan-epitopes with a miniature microarray analytical platform allowing screening of glycan occurrence in a broad range of biological contexts.

Detecting Circulating Anti-Glycan Antibodies with a Printed Glycan Array

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2025

This video demonstrates printed glycan array technology for analyzing circulating anti-glycan antibodies in mouse serum. The antibodies in the serum interact with specific glycans on the chip, which is confirmed by immunostaining followed by fluorescence scanning.

Glycan Node Analysis: A Bottom-up Approach to Glycomics

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Cited by 16 •

2016

This article presents an enhanced form of a novel bottom-up glycomics technique designed to analyze the pooled compositional profile of glycans in unfractionated biofluids through the chemical breakdown of glycans into their constituent linkage-specific monosaccharides for detection by GC-MS. Potential applications include early detection of cancer and other glycan-affective disorders.

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

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Cited by 1 •

2016

This protocol describes the specific techniques used for the structural characterization of reducing end (RE) and internal region glycosyl sequence(s) of heteroxylans by tagging the RE with 2 aminobenzamide prior to enzymatic (endoxylanase) hydrolysis and then analysis of the resultant oligosaccharides using mass spectrometry (MS) and nuclear magnetic resonance (NMR).

Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography

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Cited by 48 •

2014

The bacterial cell wall is composed of peptidoglycan, a macromolecular network of sugar strands crosslinked by peptides. Ultra Performance Liquid Chromatography provides high resolution and throughput for novel discoveries of peptidoglycan composition. We present a procedure for the isolation of cell walls (sacculi) and their subsequent preparation for analysis via UPLC.

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