Chip-based Cell Culture
Chip-based Cell Culture - Related Videos
10:36
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
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Cited by 6 •
2012
We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.
12:39
Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
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Cited by 7 •
2008
We describe a chip-based platform for the three-dimensional cultivation of cells in micro-bioreactors. One chip can house up to 10 Mio. cells that can be cultivated under precisely defined conditions with regard to fluid flow, oxygen tension etc. in a sterile, closed circulation loop.
04:53
Compartmentalized Primary Murine Neuron Culture Using Plastic Microfluidic Chips
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2025
This video demonstrates the utilization of a pre-assembled plastic microfluidic chip for the compartmentalized culture of primary rat hippocampal neurons. The neurons are plated into one of the microfluidic compartments, where they attach and grow, extending their axons through the chip's microgrooves into the adjacent compartment. The narrow microgrooves prevent neuronal cell bodies from entering, thereby enabling physical separation between the somatic and axonal compartments.
05:25
Patterning of Embryonic Stem Cells Using the Bio Flip Chip
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Cited by 2 •
2007
We demonstrate a simple method for placing cells at desired locations on a substrate. This method patterns cells by flipping a silicone chip containing microwells filled with cells onto the substrate. This method provides a new way to modulate diffusible and juxtacrine signaling between cells.
09:37
Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation
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Cited by 11 •
2008
We present two processes for the microfabrication of porous polymer chips for three-dimensional cell cultivation. The first one is hot embossing combined with a solvent vapour welding process. The second one uses a recently developed microthermoforming process combined with ion track technology leading to a significant simplification of manufacture.
FAQs
Controlled fluid flow regulates how nutrients, oxygen, and chemical signals reach cultured cells. It can also create mechanical forces, including shear stress, that cells would encounter in particular tissue environments. By adjusting these conditions within microfluidic channels, researchers can examine cellular responses under defined gradients and physical forces rather than relying only on static culture conditions.
Extracellular matrix interactions provide environmental cues that influence how cells behave within the chip. Combining matrix-related conditions with controlled nutrients, oxygen, and chemical gradients allows researchers to study cell responses in a more contextually structured setting. This is especially useful when the goal is to model tissue behavior rather than observe cells under simplified conditions alone.
Key variables include nutrient availability, oxygen levels, chemical gradients, extracellular matrix interactions, fluid movement, and mechanical forces such as shear stress. Controlling these factors separately or together helps researchers determine which environmental conditions affect cell behavior. The resulting experimental control can clarify biological responses that might be difficult to distinguish in conventional culture systems.
Chip-based systems provide tighter control over environmental conditions while using smaller quantities of cells and reagents. Their microfluidic design can reproduce gradients, fluid movement, matrix interactions, and mechanical forces that conventional systems may represent less precisely. Real-time observation also supports direct monitoring of cellular behavior as conditions change, helping connect simplified culture findings with tissue-level biology.
A general workflow involves placing the selected cells within the engineered platform, establishing the desired fluid-flow conditions, and regulating relevant environmental factors such as nutrients, oxygen, chemical gradients, or matrix interactions. Researchers then observe cell behavior in real time under the defined conditions. The exact setup depends on the biological question and the features being modeled.
Researchers may choose this approach when they need to investigate tissue-like conditions, disease-related cell behavior, or responses to controlled chemical and mechanical environments. It is also suited to drug-screening studies that benefit from real-time observation and precise experimental control. The reduced use of cells and reagents can make such investigations more efficient than some conventional culture approaches.
These platforms can reveal how cells respond to changing nutrients, oxygen, chemical gradients, extracellular matrix conditions, fluid flow, and shear stress. They support real-time observation of those responses, making it possible to connect environmental conditions with cell behavior. In broader applications, the information can contribute to tissue modeling, disease research, drug screening, and studies of biological mechanisms.