Lucifer Yellow Dye
Lucifer Yellow Dye - Related Videos
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Yellow fever is a viral hemorrhagic disease caused by the yellow fever virus (YFV), a member of the Flaviviridae family. It is transmitted primarily by Aedes and Haemagogus mosquitoes in tropical and subtropical regions of Africa and South America. After transmission through a mosquito bite, the virus initially replicates in skin-resident immune cells such as dendritic cells and macrophages. These cells then migrate to the lymph nodes, where viral replication increases, eventually leading to...
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Assessing Pseudovirus Infection Using Luciferase Reporter Assay
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Source: Chang, X., et al. Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity. J. Vis. Exp. (2021).This video demonstrates a luciferase-based assay to quantify pseudovirus infection in epithelial cells. The pseudovirus encodes the luciferase gene, which is expressed in infected cells. After washing, and cells are incubated with lysis buffer at an ultra-low temperature, and then thawed to induce lysis.
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UV-Vis Spectroscopy of Dyes - Student Protocol
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Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA Absorbance Wavelength ComparisonExpand In the first part of the lab, you'll use ultraviolet and visible light absorption spectroscopy, or UV-Vis absorption spectroscopy, to analyze the absorption characteristics of fluorescein, β-carotene, and indigo dye. To perform UV-Vis spectroscopy, you'll place a solution between a light source and a photodetector. The molecules will absorb light at...
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Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.
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Sample Preparation for Luciferase-Based Viral Quantification
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2026
Source: Garcia, V., et al. High-throughput Titration of Luciferase-expressing Recombinant Viruses. J. Vis. Exp. (91), e51890, doi:10.3791/51890 (2014).This video demonstrates the preparation of cell culture samples to assess viral load using a luciferase-based assay. It outlines the process of infecting epithelial cells with a genetically modified virus, transferring viral supernatants and standards into assay wells, and promoting viral entry through centrifugation and incubation. The...
FAQs
Because Lucifer Yellow dye is generally membrane-impermeant, it does not readily enter cells from the surrounding medium. Researchers therefore place it directly into a selected cell using iontophoretic injection or a patch pipette. This delivery requirement restricts labeling to targeted cells and helps associate the observed fluorescence with the injected cell’s cytoplasmic structure.
Lucifer Yellow can move from one labeled cell into other cells when functional gap junctions connect them. Observing this spread provides evidence of intercellular coupling rather than merely showing the morphology of the injected cell. The dye therefore supports studies that connect cellular anatomy with communication, helping distinguish isolated cells from cells participating in coupled tissue networks.
Under fluorescence microscopy, the emitted signal makes the labeled cell and its processes visible against the surrounding tissue. Investigators can use that pattern to examine cellular morphology, trace connections, and assess how cells are arranged within a tissue. The resulting visual information is especially useful when structure must be related to connectivity or tissue organization.
Researchers select a cell, introduce Lucifer Yellow by iontophoretic injection or through a patch pipette, and examine the preparation with a fluorescence microscope. They then evaluate the visible signal for cytoplasmic structure and processes. If fluorescence extends beyond the injected cell, the pattern can also be assessed for evidence of functional gap-junction coupling.
In neuronal tracing, labeling an individual cell makes its processes visible, allowing researchers to examine how that cell’s structure extends through biological tissue. The same fluorescence pattern can also help reveal connections associated with the labeled cell. This combination of single-cell visibility and structural detail supports studies of neuronal morphology and organization.
Developmental research can use the tracer to examine cellular structure and connections within developing biological tissues. In tissue-organization studies, fluorescence helps show how individual cells and their processes are positioned within a larger arrangement. When gap-junction transfer is also observed, researchers can add information about physiological interactions and intercellular coupling to the structural picture.