Non-enzymatic Tissue Culture
Non-enzymatic Tissue Culture - Related Videos
09:49
Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres
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2014
Primary cell culture using intact tissue organoids provides a model system that mimics the multi-cellular in vivo microenvironment. We developed a serum-free primary breast epithelium tissue culture model that perpetuates mixed cell culture lineages and exhibits differentiated morphology, without enzymatic tissue disruption. Breast organoids remain viable for >6 months.
09:35
Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
02:57
Plant Tissue Culture
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2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
07:29
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
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Cited by 29 •
2014
This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.
FAQs
Retaining the starting tissue’s native organization allows cells to remain in spatial relationships that can influence development, regeneration, and cell-to-cell interactions. This structure also helps researchers observe how an intact tissue responds to environmental or chemical stimuli. The resulting model can therefore provide biological context that may be reduced when cells are studied only after dissociation.
The medium supplies essential water, mineral salts, and sugars that support tissue survival and continued cellular activity. Depending on the experimental system, plant growth regulators or other signaling factors may also be added to influence cellular behavior. Adjusting these components helps researchers examine how nutritional or signaling conditions affect division and reorganization within the cultured tissue.
The main distinction is the level of structural organization retained during culture. Non-enzymatic tissue culture maintains tissue fragments and their native arrangement, whereas dissociated-cell systems separate cells before growth. Because each model preserves different biological information, researchers can use the tissue-based approach to study interactions and responses that depend on organization, while using dissociated systems for complementary cellular analysis.
The process begins with placing a tissue fragment under aseptic conditions, meaning procedures are performed to prevent unwanted contamination. The fragment is then positioned on a nutrient medium containing water, mineral salts, and sugars, with signaling factors added when needed. During maintenance, researchers observe whether the tissue survives, divides, and reorganizes within the culture.
Researchers may choose this approach when the experimental question depends on tissue organization rather than isolated-cell behavior. It is useful for examining tissue development, regeneration, cell interactions, or responses to environmental and chemical stimuli. Comparing results with dissociated-cell systems can help distinguish effects that arise from individual cells from those associated with their surrounding tissue structure.
Cultured tissue can reveal whether cells remain viable, undergo division, and reorganize under defined nutritional or signaling conditions. These observations support studies of developmental patterns, regenerative behavior, cellular interactions, and stimulus responses. In biology research, the method therefore provides a tissue-level experimental model that connects cellular activity with changes occurring within an organized fragment.