Journal
/
Biology
/
Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres

Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres

DOI:
10.3791/51926-v

•

09:49 min

•

November 08, 2014

•

, ,
1Center for Applied Proteomics and Molecular Medicine,George Mason University, 2Virginia Surgery Associates

Chapters

  • 00:05Title
  • 02:03Tissue Acquisition and Grossing
  • 04:22Maintenance of Established Organoid/Epithelial Cell Colonies
  • 06:43Results: Representative Mammosphere Imaging
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

Primary cell culture using intact tissue organoids provides a model system that mimics the multi-cellular in vivo microenvironment. We developed a serum-free primary breast epithelium tissue culture model that perpetuates mixed cell culture lineages and exhibits differentiated morphology, without enzymatic tissue disruption. Breast organoids remain viable for >6 months.

Tags

Keywords: Non-enzymaticSerum-freePre-invasive Breast LesionsSpontaneous GenerationMammospheresBreast Ductal Carcinoma In Situ (DCIS)Progenitor CellsInvasionOrganoidsEpithelial CellsFibroblastsCytogenetically AbnormalInvasive Progenitor Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51926?article_type=v

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
check_url/51926?article_type=v

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation
check_url/51926?article_type=v

Generation of Single-Cell Suspensions from Mouse Neural Tissue
check_url/51926?article_type=v

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
check_url/51926?article_type=v

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
check_url/51926?article_type=v

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
check_url/51926?article_type=v

Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
check_url/51926?article_type=v

Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
check_url/51926?article_type=v

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
check_url/51926?article_type=v

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

Read Article