A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

Soizic Garaud; Chunyan Gu-Trantien; Jean-Nicolas Lodewyckx; Ana&#239;s Boisson; Pushpamali De Silva; Laurence Buisseret; Edoardo Migliori; Myriam Libin; C&#233;line Naveaux; Hugues Duvillier; Karen Willard-Gallo

doi:10.3791/52392

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A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

19,923 Views

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07:29 min

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December 06, 2014

DOI:

10.3791/52392-v

DOI:

10.3791/52392-v

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07:29 min

December 06, 2014

•

11 Views

Soizic Garaud*^1,2, Chunyan Gu-Trantien*^1,2, Jean-Nicolas Lodewyckx^1,2, Anaïs Boisson^1,2, Pushpamali De Silva^1,2, Laurence Buisseret^1,2, Edoardo Migliori^1,2, Myriam Libin³, Céline Naveaux^1,2, Hugues Duvillier^1,4, Karen Willard-Gallo^1,2

¹Molecular Immunology Unit,Université Libre de Bruxelles, ²Institut Jules Bordet,Université Libre de Bruxelles, ³Institut d’Immunologie Médicale,Université Libre de Bruxelles, ⁴Flow Cytometry Core Facility,Université Libre de Bruxelles

Summary

This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45⁺ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.