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A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

DOI:
10.3791/52392-v

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07:29 min

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December 06, 2014

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, , , , , , , , , ,
1Molecular Immunology Unit,Université Libre de Bruxelles, 2Institut Jules Bordet,Université Libre de Bruxelles, 3Institut d’Immunologie Médicale,Université Libre de Bruxelles, 4Flow Cytometry Core Facility,Université Libre de Bruxelles

Chapters

  • 00:05Title
  • 01:47Preparation of the Tissue Homogenate
  • 04:15Separation and Clarification of the Tissue Supernatant
  • 05:37Results: Tumor Infiltrating Lymphocyte (TIL) Subsets in the Homogenate
  • 07:00Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.

Tags

Lymphocyte IsolationTumor Infiltrating LymphocytesBreast CancerImmune ResponseFlow CytometryProtein AnalysisCytokinesChemokines

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