Mouse Fetal Intestine
Mouse Fetal Intestine - Related Videos
08:15
Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids
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Cited by 7 •
2019
This protocol describes how to mimic suckling-to-weaning transition in vitro using mouse late fetal intestinal organoids cultured for 30 days.
09:20
A Mouse Fetal Skin Model of Scarless Wound Repair
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Cited by 24 •
2015
During mammalian development, early gestational skin wounds heal without a scar. Here we detail a reliable and reproducible model of fetal scarless wound healing in the cutaneous dorsum of E16.5 (scarless) and E18.5 (scarring) mouse embryos.
06:55
Ileectomy-induced Bile Overaccumulation in Mouse Intestine
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Cited by 4 •
2017
Small intestine-dependent bile acid reabsorption and feedback inhibition of hepatic bile acid synthesis is important for systemic homeostasis and health. In this study, we describe a mouse model for ileal resection to evaluate ileectomy-induced bile malabsorption, overaccumulation, and toxicity in mouse intestine.
10:12
A Novel Surgical Approach for Intratracheal Administration of Bioactive Agents in a Fetal Mouse Model
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Cited by 8 •
2012
We developed a novel surgical approach for intratracheal administration of bioactive agents into the mouse fetus. The delivery route is more efficient in targeting the fetal mouse lungs than the commonly used intra-amniotic injection. This procedure has to date not been described in a mouse model.
09:47
Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
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Cited by 13 •
2015
The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.
FAQs
These processes act together rather than independently. Cell proliferation expands the developing tissue, differentiation produces specialized cell populations, migration positions cells within the forming gut, and tissue folding establishes organized intestinal regions. Their coordination helps transform the primitive gut tube into a structure with aligned epithelial, connective, muscular, neural, and vascular components suitable for later gastrointestinal function.
Intestinal maturation depends on interactions among several tissue systems. The epithelium contributes to future digestion, absorption, and barrier activity, while connective tissue, smooth muscle, and developing neural and vascular networks provide structural and organizational context. Examining these components together reveals how coordinated tissue development supports epithelial maturation and the formation of an integrated gastrointestinal organ.
Developmental signaling helps coordinate the cellular behaviors that shape the intestine, including proliferation, differentiation, migration, and folding. Changes in these regulatory influences may alter regional organization or the maturation of intestinal tissues. Studying signaling in the fetal organ therefore connects molecular regulation with tissue architecture and helps explain how normal gastrointestinal development can be disrupted.
Investigators can examine how the primitive gut becomes regionally organized and how intestinal tissues mature during gestation. Relevant observations include changes in cell proliferation, differentiation, migration, tissue folding, epithelial maturation, and the development of neural and vascular networks. Together, these features provide a developmental picture that links cellular behavior with the emergence of gastrointestinal structure.
Because its development includes coordinated formation of intestinal regions and supporting tissue systems, the mouse fetal intestine provides a model for examining developmental abnormalities. Researchers can consider how altered genetic or environmental influences affect cellular behavior, epithelial maturation, tissue organization, or network development. These comparisons can help relate changes during organ formation to congenital gastrointestinal disorders.
Developmental intestinal tissue offers a context for studying how epithelial cells mature while the surrounding organ architecture is forming. This is relevant to regeneration research because normal development provides information about tissue organization and epithelial change. The model also supports investigation of how developmental signaling, genetic factors, or environmental conditions influence gastrointestinal biology before and after birth.