Stem Cell Line Derivation
Stem Cell Line Derivation - Related Videos
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 7 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
11:56
Derivation of Human Embryonic Stem Cells by Immunosurgery
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Cited by 12 •
2007
The ability of human embryonic stem cells to self-renew and differentiate into all cell types of the body suggests that they hold great promise for both medical applications and as a research tool for addressing fundamental questions in development and disease. Here, we provide a concise, step-by-step protocol for the derivation of human embryonic stem cells from embryos by immunosurgical isolation of the inner cell mass.
10:33
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Cited by 24 •
2014
Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.
12:21
Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
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Cited by 12 •
2016
This work describes an in vitro differentiation protocol to produce pigmented, mature melanocytes from human pluripotent stem cells via a neural crest and melanoblast intermediate stage using a feeder-free, 25 day protocol.
12:59
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
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Cited by 1 •
2017
This article describes a protocol to efficiently derive and culture pluripotent stem cell lines from mouse embryos at the blastocyst stage.
FAQs
Defined culture conditions determine whether suitable cells survive and continue self-renewing, meaning they produce additional cells while maintaining their properties. When pluripotency is required, the conditions must also support that capability. This makes the culture environment central to establishing a stable population rather than merely keeping cells alive for a short period.
The starting material can come from an embryo, adult tissue, or reprogrammed somatic cells. This choice determines the biological origin of the resulting model and helps align the line with its intended use. For example, researchers may select among these sources when designing studies of development, disease mechanisms, drug responses, or regenerative biology.
Expansion alone does not show that a culture has retained the intended biological properties. Characterization examines the line's identity and functional properties, helping researchers determine whether it represents the desired stem cell population. These assessments support confident use of the cells in developmental, disease, drug-response, and tissue-engineering studies.
The process begins by placing suitable cells under defined conditions that support survival and self-renewal. Researchers then expand the population to create renewable material and characterize its identity and functional properties. If the intended model requires pluripotency, the culture conditions and subsequent characterization must address that property as part of the workflow.
A well-characterized line provides a renewable population for investigating disease mechanisms and examining responses to drugs. Because the cells can be maintained and expanded, researchers can obtain material for repeated studies rather than relying on a single limited sample. The line's identity and functional properties remain important for interpreting those experimental outcomes.
Derived lines supply renewable material for regenerative biology and tissue engineering research. Their value comes from combining continued laboratory maintenance with expansion into a larger available population. Depending on the source and properties established during characterization, a line can serve as a consistent biological model while researchers investigate applications related to tissue formation and repair.