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Chapters
- 00:07Introduction
- 01:17Preparation of plates for immunosurgery
- 02:34Preparing capillary pipettes
- 03:32Immunosurgery to isolate Inner Cell Mass (ICM)
- 06:30Development of human ES cell outgrowth
- 06:42Passaging outgrowth
- 07:49Discussion: Technical aspects of human ES cell derivation
- 09:12Heterogeneity of human ES cell lines: implications for development
- 10:53Advances in human ES cell derivation
The ability of human embryonic stem cells to self-renew and differentiate into all cell types of the body suggests that they hold great promise for both medical applications and as a research tool for addressing fundamental questions in development and disease. Here, we provide a concise, step-by-step protocol for the derivation of human embryonic stem cells from embryos by immunosurgical isolation of the inner cell mass.
