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Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells

Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells

DOI:
10.3791/53806-v

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12:21 min

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March 03, 2016

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, ,
1The Center for Stem Cell Biology, Developmental Biology Program,Memorial Sloan-Kettering Cancer Center, 2Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School,Sloan-Kettering Institute for Cancer Research, 3Thermo Fisher Scientific

Chapters

  • 00:05Title
  • 00:42Coating of Culture Dishes
  • 01:50Maintenance of Human Pluripotent Stem Cells
  • 03:22Plating hPSCs for Differentiation
  • 05:27Induction of Neuronal Differentiation
  • 07:49Replating in Droplets for Neural Crest Specification
  • 09:31Expanding Melanocyte Progenitors
  • 10:21Results: Derivation of Mature Melanocytes from hPSCs Using a Feeder-free Method
  • 11:47Conclusion

Summary

Automatic Translation

Automatic Translation

This work describes an in vitro differentiation protocol to produce pigmented, mature melanocytes from human pluripotent stem cells via a neural crest and melanoblast intermediate stage using a feeder-free, 25 day protocol.

Tags

Keywords: MelanocytePluripotent Stem CellsPigmentationFeeder-freeDifferentiationDrug DiscoveryDisease ModelingCulture ConditionsLamininFibronectinMouse Embryonic FibroblastsHESC Medium

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