Embryo Tissue Processing
Embryo Tissue Processing - Related Videos
08:19
Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo
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Cited by 11 •
2011
This article describes surface labeling and ex ovo tissue culture in the early chick embryo. Techniques amenable to time-lapse bright field, fluorescence, and optical coherence tomography imaging are presented. Tracking surface labels with high spatiotemporal resolution enables kinematic quantities such as morphogenetic strains (deformations) to be calculated in both two and three dimensions.
07:06
Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos
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Cited by 6 •
2014
Described here is a protocol for semi-automated imaging of tissue-specific fluorescence in zebrafish embryos.
11:02
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
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Cited by 5 •
2011
We demonstrate a chromatin immunoprecipitation (ChIP) method to identify factor interactions at tissue-specific genes during or after the onset of tissue-specific gene expression in mouse embryonic tissue. This protocol should be widely applicable for the study of tissue-specific gene activation as it occurs during normal embryonic development.
15:52
Postproduction Processing of Electrospun Fibres for Tissue Engineering
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Cited by 30 •
2012
Electrospun scaffolds can be processed post production for tissue engineering applications. Here we describe methods for spinning complex scaffolds (by consecutive spinning), for making thicker scaffolds (by multi-layering using heat or vapour annealing), for achieving sterility (aseptic production or sterilisation post production) and for achieving appropriate biomechanical properties.
14:21
High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
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Cited by 25 •
2017
The described RNA in situ hybridization protocol allows the detection of RNA in whole Drosophila embryos or dissected tissues. Using 96-well microtiter plates and tyramide signal amplification, transcripts can be detected at high resolution, sensitivity, and throughput, and at a relatively low cost.
FAQs
Fixation stabilizes the specimen before later handling, helping preserve embryonic tissue architecture as dehydration, embedding, and sectioning proceed. This preservation is essential because developmental structures must remain interpretable during microscopic analysis. Reliable fixation therefore supports examination of cell organization, tissue differentiation, morphogenetic changes, and abnormalities within the prepared embryo.
Dehydration removes water, embedding provides support, and sectioning produces thin slices suitable for microscopy. Their sequence converts a preserved embryo into sections where structural relationships can be examined at cellular and tissue scales. Together, these stages help researchers interpret both local organization and broader changes in embryonic form within the same preparation.
Staining makes particular tissue features more visible under microscopic examination, while immunostaining supports analysis of selected biological signals within the preparation. These approaches add interpretive detail beyond tissue architecture alone. In developmental biology, they help relate visible patterns of organization and differentiation to gene activity and cellular behavior during embryonic development.
Prepared embryos allow researchers to examine how cells are organized, how tissues differentiate, and how form changes through morphogenesis. They can also reveal developmental abnormalities that may not be explained by overall appearance alone. Comparative analysis of these features helps connect cellular arrangements with the changing structure of the developing embryo.
The workflow proceeds through fixation, dehydration, embedding, sectioning, and staining or immunostaining. Each stage prepares the specimen for the next while preserving access to structural or biological information. Maintaining this progression helps produce sections that can be visualized consistently, allowing researchers to assess tissue architecture and developmental features without losing the relationships established in the embryo.
Histology and immunostaining provide complementary views of prepared embryonic tissues, while comparative analyses relate those observations to developmental morphology. By examining tissue organization alongside signals associated with gene activity or cellular behavior, researchers can interpret how microscopic processes correspond to visible changes in embryonic structure. This connection is central to developmental biology studies of morphogenesis and differentiation.