Cell Surface Profiling
Cell Surface Profiling - Related Videos
15:17
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Cited by 19 •
2011
We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.
10:20
Single-cell Profiling of Developing and Mature Retinal Neurons
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Cited by 13 •
2012
A method for the isolation of single retinal cells and subsequent amplification of their cDNAs is described. Single-cell transcriptomics reveals the degree of cellular heterogeneity present in a tissue and uncovers new marker genes for rare cell populations. The accompanying protocol can be adjusted to suit many different cell types.
14:11
Quantification of Hydrogen Concentrations in Surface and Interface Layers and Bulk Materials through Depth Profiling with Nuclear Reaction Analysis
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Cited by 21 •
2016
We illustrate the application of 1H(15N,αγ)12C resonant nuclear reaction analysis (NRA) to quantitatively evaluate the density of hydrogen atoms on the surface, in the volume, and at an interfacial layer of solid materials. The near-surface hydrogen depth profiling of a Pd(110) single crystal and of SiO2/Si(100) stacks is described.
08:49
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Cited by 8 •
2020
This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand interactions.
01:21
Cell-surface Signaling
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2019
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects. Variety of Receptor Families
FAQs
Signal intensity reflects the amount of detected surface marker associated with individual cells. Comparing these signals across measured cells can reveal differences between populations rather than treating all marker-positive cells as equivalent. In immunology, this supports more precise separation of immune-cell subsets and helps identify changes in activation or exhaustion states.
Labeled antibodies provide the molecular recognition needed to detect selected surface markers, while flow cytometry or mass cytometry measures those signals at the single-cell level. These approaches generate complementary profiles of cell populations and marker abundance. Related single-cell methods can extend the analysis when researchers need a molecular snapshot of cellular identity or state.
Surface receptors can influence how pathogens attach to or enter host cells, making their distribution biologically important in infection research. Profiling these molecules helps researchers compare susceptible and less-susceptible cell populations and examine host-pathogen interactions. The resulting patterns can also highlight receptors or receptor-bearing cells relevant to targeted diagnostic or therapeutic development.
A study generally selects surface markers relevant to the immune question, detects them with labeled antibodies or a related single-cell method, and measures the resulting signals across cells. Researchers then compare marker patterns among populations or conditions. This workflow connects molecular measurements with cell identity, activation or exhaustion, and interactions relevant to infection.
It is particularly useful when researchers need to distinguish immune-cell subsets or follow changes in immune status. Profiles can reveal activation or exhaustion patterns and help track immune responses in infection-related studies. Because the measurements are made across individual cells, the approach can expose differences among populations that would be obscured in a combined sample.
The resulting molecular patterns can support biomarker discovery, clarification of host-pathogen interactions, and monitoring of immune responses. They may also identify cell-surface features useful for targeted diagnostics or therapies. In this way, profiling connects descriptive measurements of surface molecules with practical questions about disease-related cell states and potential intervention targets.