Dendritic Cell Transfection
Dendritic Cell Transfection - Related Videos
08:08
Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
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Cited by 11 •
2011
We present our optimized high-throughput nucleofection protocol as an efficient way of transfecting primary human monocyte-derived dendritic cells with either plasmid DNA or siRNA without causing cell maturation. We further provide evidence for successful siRNA silencing of targeted gene RIG-I at both the mRNA and protein levels.
16:04
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
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Cited by 6 •
2013
Here, we use retroviral transduction and concatemeric transfection to create a cell line that can express the components of a lentiviral vector (LV) in the absence of tetracycline. This LV encodes GFP and is pseudotyped with a glycoprotein, SVGmu, which is specific for a receptor on dendritic cells.
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
05:26
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
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Cited by 5 •
2011
We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies.
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
FAQs
Electroporation and lipid-based carriers provide alternative routes for moving nucleic acids across the dendritic cell membrane. Their shared purpose is to make DNA, RNA, or other nucleic acids accessible inside the cell, where the introduced material can influence gene expression. Comparing these delivery approaches helps researchers select a strategy suited to studying immune regulation or directing a desired response.
The introduced nucleic acid determines which cellular process researchers can examine or direct. DNA or RNA may support production of a selected protein, while other nucleic-acid designs can silence a target gene or modify antigen-processing pathways. This choice connects the transfection experiment to specific questions about dendritic-cell function, immune signaling, or antigen handling.
Altering antigen-processing pathways can change how dendritic cells handle immune-relevant material and influence subsequent antigen presentation. Because dendritic cells help connect innate and adaptive immunity, transfection provides a way to examine how pathway changes affect T-cell activation and broader immune responses. This makes antigen processing a useful focus in mechanistic immunology and infection studies.
Transfected dendritic cells can be used to examine cytokine production, T-cell activation, and antigen presentation as distinct but related outcomes. Researchers can connect a nucleic-acid-driven change in gene expression with these immune readouts to clarify how dendritic cells shape responses. The approach therefore links intracellular molecular changes with measurable effects on immune communication.
A conceptual workflow begins by selecting DNA, RNA, or another nucleic acid according to the biological question, followed by choosing electroporation or a lipid-based carrier for delivery. Researchers then evaluate the resulting change in gene expression, protein production, gene silencing, or antigen-processing pathways. The selected immune outcomes determine how the experiment is interpreted.
In infection research, this approach helps investigate pathogen-associated responses by changing selected functions in dendritic cells and examining the resulting immune effects. Researchers can use the system to connect altered gene expression or antigen processing with cytokine production, antigen presentation, or T-cell activation. These observations help clarify how dendritic cells participate in immune responses to infection.
The method supports vaccine research by allowing investigators to study or direct dendritic-cell activities linked to antigen processing, antigen presentation, and T-cell activation. Transfected cells can therefore serve as an experimental system for examining how selected nucleic-acid-driven changes influence immune responses relevant to vaccination. The resulting data can clarify mechanisms underlying candidate vaccine strategies.
In cellular immunotherapy research, transfection can be used to alter dendritic-cell gene expression or antigen-processing pathways before evaluating immune effects. Researchers may examine whether these changes influence cytokine production, antigen presentation, or T-cell activation. This provides a way to investigate how modified dendritic cells could direct immune responses in therapeutic research, while preserving focus on defined cellular mechanisms.