Sirna Electroporation
Sirna Electroporation - Related Videos
03:04
siRNA Encapsulation into Exosomes Using Electroporation: An Electric Pulse Based Technique to Load siRNA into Exosomes
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2023
This video describes an electroporation-based method for encapsulating siRNA into exosomes. The siRNA-loaded exosomes can then be used for therapeutic applications.
02:30
siRNA - Small Interfering RNAs
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2020
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements. In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
03:44
siRNA-Based Inhibition of Autophagy in Herpes Simplex Virus-Infected Immature Dendritic Cells
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2025
This video demonstrates a small interfering RNA (siRNA)-based technique to interfere with autophagic flux. A siRNA targeting FIP200 mRNA is introduced into monocyte-derived immature dendritic cells (iDCs) using electroporation, causing the knockdown of FIP200 expression and leading to the inhibition of nuclear lamin disruption via autophagy. Upon adding herpes simplex virus (HSV), the nuclear egress of viral progeny is inhibited due to the absence of lamin disruption via autophagic flux.
09:14
High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)
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Cited by 4 •
2012
The article details the protocol for site-specific transfection of scrambled sequence of siRNA in an adherent mammalian cell culture using a microelectrode array (MEA).
10:33
Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
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Cited by 18 •
2016
The goal of this protocol is to use cationic/anionic liposomes with a neuro-targeting peptide as a CNS delivery system to enable siRNA to cross the BBB. The optimization of a delivery system for treatments, like siRNA, would allow for more treatment options for prion and other neurodegenerative diseases.
FAQs
Brief electrical pulses increase membrane permeability, creating a temporary delivery window through which siRNA can enter cells. The electrical treatment enables uptake but does not provide the gene-silencing specificity itself. After entry, the siRNA directs sequence-matched messenger RNA degradation through the RNA-induced silencing complex, allowing targeted suppression without permanent genome modification.
Sequence complementarity determines which messenger RNA is recognized by the RNA-induced silencing complex. This matching links the introduced siRNA to a particular transcript rather than broadly suppressing protein production. As a result, investigators can examine the consequences of reducing a selected host or pathogen-related gene and relate that change to immune or infection-associated phenotypes.
siRNA electroporation produces transient gene suppression because it acts through messenger RNA degradation rather than changing the cell's genomic DNA. This distinction allows researchers to study the short-term consequences of reducing a gene while avoiding permanent genome modification. The approach is therefore useful when reversible functional perturbation is preferable to stable genetic alteration.
A general workflow combines cells, a selected siRNA, and an electroporation step that briefly increases membrane permeability. Following delivery, the introduced siRNA engages the RNA-induced silencing complex and reduces the corresponding messenger RNA and protein production. Investigators can then examine how the targeted reduction affects the cellular process under study, including immune or infection-related responses.
The method is particularly valuable for difficult-to-transfect cells, where conventional delivery may not efficiently introduce siRNA. Researchers can suppress host genes to study immune signaling, inflammatory responses, or pathogen entry, or target pathogen-related genes to investigate replication. These applications connect a selected gene to a measurable infection or immune phenotype through transient functional analysis.
By reducing production of a selected protein, siRNA electroporation supports functional studies of pathways involved in immunity and infection. Changes in pathogen entry, replication, signaling, or inflammation can help reveal whether the targeted gene contributes to those processes. The resulting evidence can also support evaluation of potential therapeutic targets without requiring permanent genomic modification.