Spiral Plating Method
Spiral Plating Method - Related Videos
02:35
Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays
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2025
This video demonstrates the process for isolating spiral ganglion explants from the inner ear and co-culturing them with the organ of Corti on a multielectrode array. Spiral ganglia are carefully extracted and sectioned into smaller tissue sections or explants. These explants are cultured on the multielectrode array's active area to promote neuronal process outgrowth.
07:51
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays
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Cited by 5 •
2016
We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
03:51
Label-Free Neutrophil Separation from Tracheal Secretion Using Spiral Microfluidics Channel
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2025
This video demonstrates separating neutrophils from tracheal secretions using a microfluidic device with spiral channels. The device achieves efficient separation based on cell size by utilizing varying thicknesses and trapezoidal-shaped channels. The interplay between forces results in large neutrophils accumulating near the inner wall while erythrocytes and mucin aggregates remain near the outer wall, ensuring effective neutrophil separation.
05:14
Differentiating Immortalized Multipotent Otic Progenitors into Spiral Ganglion Neurons and Evaluating the Differentiation
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2025
The video demonstrates the differentiation of immortalized multipotent otic progenitors (iMOPs) into spiral ganglion neurons (SGNs) and immunofluorescence-based confirmation of the differentiation. The iMOPs are plated onto culture substrate-coated coverslips in a neuronal differentiation medium for differentiation into SGNs. The differentiated cells are labeled with antibodies specific for neuronal differentiation markers and analyzed under a microscope to confirm differentiation.
FAQs
The controlled spiral deposition places different portions of the measured sample at progressively different concentrations across the agar surface. This creates regions where colonies may be sufficiently separated for counting, even when a single uniform distribution would produce too many colonies in one area. Counts from suitable regions can then support estimation of viable microorganisms as colony-forming units.
Plate rotation and coordinated movement of the motorized stylus or dispensing arm determine where the liquid sample is deposited. Their controlled motion produces the spiral pattern rather than an uneven manual spread. Because the instrument delivers a measured volume while the plate rotates, the resulting distribution can be related to colony counts across distinct areas of the agar.
Both approaches distribute a liquid sample on agar for viable microorganism enumeration, but spiral plating uses a controlled gradient rather than a uniform surface spread. This design can reduce the amount of media and sample required while increasing processing efficiency. The method therefore supports higher throughput when many samples must be examined for microbial counts.
The essential setup includes an agar plate, a liquid sample, and an instrument with a motorized stylus or dispensing arm. The plate must rotate while the instrument deposits a measured volume in a controlled spiral pattern. These components work together to create the gradient needed for obtaining countable colonies and calculating colony-forming units.
The method can quantify bacteria in clinical, environmental, and experimental samples. In infection-related research, those measurements can support investigations of microbial growth, contamination, and changes associated with antimicrobial treatments. Its ability to process samples with relatively low media and sample use also makes it useful when researchers need efficient analysis across multiple conditions.
Researchers use colony counts from the agar regions to estimate viable bacteria as colony-forming units. Comparing these estimates among samples associated with antimicrobial treatments can provide a quantitative measure of bacterial recovery or persistence. In immunology and infection research, that information helps connect treatment conditions with changes in microbial burden, while also supporting broader growth and contamination assessments.