Gfp Guided Surgery
Gfp Guided Surgery - Related Videos
00:08
Survivable Stereotaxic Surgery in Rodents
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Cited by 50 •
2008
The monitoring of extracellular neurotransmitter levels in distinct brain regions of freely moving animals offers insights on the link between neurotransmitter release and behavior. In vivo microdialysis coupled with electrochemical detection provides excellent anatomical and chemical resolution; and information on how basal neurotransmission is altered by pharmacological or physiological manipulations.
07:13
Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation
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Cited by 4 •
2017
Mice with acquired hypoparathyroidism would be useful for studying novel drug therapies for hypoparathyroidism. Two procedures to create such mice are demonstrated. The GFP-PTX mouse is generated by surgical parathyroidectomy guided by green fluorescing parathyroid glands. A second, non-surgical approach is based on parathyroid-specific expression of the diphtheria toxin receptor.
08:28
Rodent Stereotaxic Surgery
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2023
Stereotaxic (or stereotactic) surgery is a method used to manipulate the brain of living animals. This technique allows researchers to accurately target deep structures within the brain through the use of a stereotaxic atlas, which provides the 3D coordinates of each area with respect to anatomical landmarks on the skull. After the skull is exposed, anesthetized animals are mounted on a specialized instrument known as a stereotaxic frame, which enables the precise placement of experimental...
10:58
Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
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Cited by 14 •
2011
This protocol describes how to perform cell viability and fluorescence expression assays using the Tali Image-Based Cytometer.
12:13
Considerations for Rodent Surgery
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN The Guide for the Care and Use of Laboratory Animals1 dictates that rodent survival surgery be performed aseptically. Aseptic technique utilizes specific practices that minimize the contamination of the surgical site, including patient preparation, surgeon preparation, sterilization of instruments and other supplies, and the use of a clean and controlled environment. Presurgical planning,...
FAQs
The key advantage comes from contrast: GFP-labeled cells emit a detectable fluorescent signal under suitable illumination, while surrounding unlabeled structures provide less signal in the same image. This difference helps the imaging system distinguish target tissue during the operation, allowing visualization to supplement anatomical landmarks. In turn, researchers can assess how well the approach supports real-time surgical decision-making in experimental settings.
Cell engineering determines which cells produce the GFP signal, making the fluorescence useful for identifying selected tissue rather than illuminating the surgical field uniformly. When engineered cells express GFP, specialized imaging can associate the observed signal with the labeled population. This feature supports targeted visualization of tumors, other tissues, or experimental therapeutic cells in preclinical medical research.
Anatomical landmarks provide structural guidance, but they may not directly identify the specific cells or tumor tissue under investigation. GFP-guided surgery adds a fluorescence-based signal that can distinguish labeled tissue from nearby structures in real time. The two approaches can therefore work together, with fluorescence supplying biological contrast while anatomy provides the surrounding spatial context.
A typical workflow begins with cells or tissue engineered to express GFP, followed by surgical illumination at a wavelength suitable for detecting the fluorescent signal. A specialized imaging system then captures the operative field and distinguishes labeled regions from surrounding tissue. Researchers can use this real-time visualization to evaluate the surgical approach, tissue location, or experimental treatment.
In tumor studies, GFP labeling can make the tumor region more visually distinguishable from surrounding tissue during an operation. The resulting fluorescence image may help researchers locate the tumor and examine the relationship between labeled tissue and the area being removed. This supports investigation of surgical margins, meaning the tissue boundary evaluated around the targeted tumor region.
GFP-guided surgery can help researchers monitor where experimental cell therapies are located when the therapeutic cells have been engineered to express GFP. In preclinical models, fluorescence provides a way to visualize these labeled cells during an operation and assess their relationship to surrounding tissues. This application helps evaluate image-guided treatment strategies before broader medical investigation.