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Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation
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Medicine
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JoVE Journal Medicine
Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation

Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation

DOI:
10.3791/55010-v

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07:13 min

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March 14, 2017

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, , , ,
1Endocrine Unit, Massachusetts General Hospital,Harvard Medical School, 2West China School of Stomatology,Sichuan University, 3Department of Oral Medicine, Infection and Immunity,Harvard School of Dental Medicine, 4State Key Laboratory of Oral Diseases, West China Hospital of Stomatology,Sichuan University

Chapters

  • 00:05Title
  • 01:18Parathyroidectomy Using GFP Glands
  • 04:08Diphtheria-Toxin-Mediated Parathyroid Ablation
  • 04:50Results: Phenotypes of 2 Acquired Hypoparathyroidism Mouse Models
  • 06:16Conclusion

Summary

Automatic Translation

Automatic Translation

Mice with acquired hypoparathyroidism would be useful for studying novel drug therapies for hypoparathyroidism. Two procedures to create such mice are demonstrated. The GFP-PTX mouse is generated by surgical parathyroidectomy guided by green fluorescing parathyroid glands. A second, non-surgical approach is based on parathyroid-specific expression of the diphtheria toxin receptor.

Tags

Keywords: ParathyroidectomyGFPDiphtheria ToxinParathyroid AblationHypoparathyroidismMouse ModelThyroid FunctionPTH Analog TherapyBone Strength

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