Kidney Procurement
Kidney Procurement - Related Videos
05:36
Kidney Procurement in a Preclinical Large Animal Model
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2025
Here, we present a protocol that details a surgical model for kidney procurement in a preclinical swine model for subsequent machine perfusion or transplantation.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
01:29
E-procurement - Buying on the Internet
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2025
Integrating information technology into business-to-business (B2B) marketing has significantly transformed how companies interact and transact. A crucial development in this domain is e-procurement, which has become standard practice for many companies. E-procurement allows buyers to access new suppliers, reduce costs, and expedite order processing and delivery. This method includes strategies such as reverse auctions, online trading exchanges, and dedicated purchasing websites, each providing...
01:45
Kidney Structure
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2019
The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water and electrolytes of the circulatory system via the renal veins. The kidneys receive blood directly from the heart via the renal arteries. These arteries enter the kidney at the hilum, the concave surface of the bean, where they branch and divide into smaller vessels and capillaries. The renal cortex is the thick outer layer of the kidney. It...
10:42
Normothermic Machine Perfusion of Rat Kidneys for Transplantation
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2026
This protocol describes the ex vivo normothermic machine perfusion of a rat kidney for subsequent transplantation into a recipient rat.
FAQs
Flushing the recovered kidney with a cold preservation solution lowers metabolic activity while the organ is outside the donor’s circulation. This reduces oxygen demand and helps limit ischemic injury, which can threaten graft viability. Maintaining hypothermic conditions during transport extends the preservation process until implantation or connection to a machine perfusion system.
These structures are essential for evaluating and preparing the kidney for transplantation. The renal artery and vein provide the vascular connections needed during implantation, while the ureter supports urinary drainage. Preserving them with the surrounding tissue also allows surgeons to assess the organ’s anatomy and vascular condition before transplantation.
The interval between donor recovery, preservation, transport, and implantation affects exposure to ischemic conditions, so careful timing helps protect graft quality. Sterile technique reduces contamination during dissection, flushing, handling, and packaging. Together with vascular assessment, these measures support organ viability and help create better conditions for successful transplantation.
Hypothermic transport preserves the kidney by maintaining low temperature after cold flushing, reducing metabolic activity during movement to the transplant center. Machine perfusion instead connects the organ to a system that supports preservation while it is being assessed or maintained. Both approaches are used to protect graft quality before implantation, but they rely on different preservation conditions.
The process begins after donor death has been determined and consent has been confirmed. Surgeons then use sterile technique to dissect the kidney, recover it with the renal artery, renal vein, ureter, and surrounding tissue, and evaluate its vascular condition. They flush the organ with cold preservation solution before hypothermic transport or machine perfusion.
Recovering a viable donor kidney provides the organ needed for transplantation, an important treatment pathway for people with end-stage renal disease. Procurement quality directly influences whether the graft remains suitable for implantation. Careful recovery, preservation, and transport therefore connect donor surgery with recipient treatment and can support improved transplant outcomes.